Enterobacter

Professor, I found that in order to identify and distinguish any form of bacteria such as Serratia sp. and Enterobacter sp. a number of assessments should be conducted. Majority of bacteria’s has enzymes allowing it to break down nucleic acids and the bacteria and utilize the resulting nucleotides to produce their own nucleic acids. DNase is an enzyme that hydrolyzes DNA. The existence of DNase entails certain strains/species of bacteria and may be utilized for typing. A DNase assessment is…

and severe cramping which is a gastrointestinal infection. It was also mentioned that raw bean sprouts were possibly the culprit for this and people were warned not to consume them. Although it can cause GI distress in some as mentioned earlier, Enterobacter aerogenes is a hospital acquired infection. This would mean that all the cases that were reported would have had to have been associated with a recent hospital stay. The main ways to acquire this in the hospital is when you are in the…

I. Purpose: The purpose of this lab was to learn how to properly mount a bacteria for examination under a microscope. Also learned in this lab were methods on how to inoculate an agar or broth medium, and using aseptic techniques. Lastly, how to use stains to visualize bacterial cells, or distinguish different types of cells from one another through the usage of dye techniques such as the Gram-stain test was learned. II. Prepping/Mounting/Simple Staining Bacterial Cells Procedure: 1. Obtain 3…

agar turned blue while we identified our unknown bacterium as Enterobacter cloacae, a clinically gram negative, facultative-anaerobic, rod shaped and motile bacterium. E. cloacae infections can include bacteremia, lower respiratory tract infections, skin and soft-tissue infections, urinary tract infections (UTIs), endocarditis, intra-abdominal infections, septic arthritis, osteomyelitis, CNS infections, and ophthalmic infections. Enterobacter infections can necessitate prolonged hospitalization,…

Unknown #29 was Enterobacter aerogenes. But for good measure as a Final test I used the Methyl Red-Vogues Proskauer test to double check my results. The MR-VP test was performed, giving the expected negative result. This indicated that the unknown did not produce acids as a byproduct of glucose metabolism. The negative result eliminated K. pneumoniae from the list of remaining possibilities. The Voges-Proskauer test demonstrated a positive result indicating that Enterobacter aerogenes was…

areas, insertion of medical devices, and during surgical operations. Infections can also transmit through fecal-oral route from person to person. Vaccines aren’t available, but antibiotics have been shown to be affective to treat E. aerogenes (Enterobacter, 2011). Since 2010, E. aerogenes has been considered to be the fifth highest Enterobacteriaceae culprit for nosocomial infections in France (Davin-Regli,…

From the patient, unknown bacteria #618 was identified to be Escherichia coli. It is apart of the Enterobacteriae genus that according to Brown’s Microbiological Applications1 are gram-negative bacteria that usually live inside the intestines of animals and humans. The organism can also live in the vagina and urethra. The data collected after isolating the organism and executing a series of tests have confirmed previous observations of E. coli in past scientific history. For the majority of…

Introduction / Purpose: Ventilator-associated pneumonia (VAP) is the most common nosocomial infection in the intensive care unit (ICU). It caused prolonged hospital stay and increased mortality. In this study, we aimed to investigate the rate of VAP, causative microorganisms and their antibiotic susceptibilities. Materials and Method: This retrospective study included patients that admitted in 12-bed ICU between January 2013 and December 2014. Centers for Disease Control and Prevention criterias…

Identification of unknown organisms is useful in a wide variety of settings; from clinical diagnostics to environmental monitoring (1). Species of bacteria can differ fundamentally in aspects ranging from morphology to metabolism. Exploiting these differences can allow for identification of unknown organisms. Gross morphological characterizations are crude, and better replaced by more accurate and specific tests. At a fundamental level, tests such as the Gram stain divide bacteria in to two…

Case Activities 1 Spotlight on AOL The facts presented in this scenario state that AOL, LLC was a brought a suit filed by 650,000 members for making their personal information public. The members alleged that AOL was in violation of California law. AOL countered by asking for dismissal of the suit based on a “forum-selection” clause that designates the Virginia courts to try member disputes. There are two precedents exposed within this problem first is that The United States Supreme Court has…