Enterobacter aerogenes

inhibitory agent towards gram-positive microorganisms and neutral red is added to serve as a pH indicator, microorganisms lower the pH which results in the colonies turning red. Some of the organisms that grow on this medium are: Escherichia coli ,Enterobacter aerogenes and Enterococcus.Lactose-fermenting organisms appear as pink or red colonies , E.coli appear pink or red, E.aerogenes appear pink in colour ,Enterococcus appear rose in colour and the colour are pin point in size…

For my first plate I decided to use an Eosin Methylene Blue. This plate gives a variety of different results that are easy to interpret. A metallic green color indicates either Escherichia coli or Citrobacter freundii, purple can indicate Enterobacter aerogenes, and a pink color occurs when Serratia marcescens is plated on an Eosin Methylene Blue plate. Upon entering the lab, I discovered metallic green colonies on the plate. I then knew I had either Escherichia coli or Citrobacter freundii.…

Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done…

gelatin hydrolysis, which ultimately allowed me to determine my microbe from another. By ignoring my MRVP test results (mostly because I was pretty sure one of them was wrong) I was able to match my results with the literature results of my unknown: Enterobacter…

Objectives: The purpose of this project is to identify two separate microbial organisms from a given list, which are to be inoculated from a mixed broth culture. Multiple tests will be performed in order to eliminate all organisms from the list that do not provide significant results for the identification of the unknown specimens. Journal Entries: April 17, 2018. Today we began the unknown project. I obtained the unknown broth tube #15. I used two Tryptic Soy Agar (TSA) plates in order to…

(hospital-acquired infection caused by bacteria, fungal and viral, parasites and other pathogens). The six pathogens are Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, and several species of Enterobacter. Instead of using these ESKAPE pathogens in classroom laboratories that are dangerous to human, we use their safe relatives that does not bring a huge risk to the human life. Safe relatives have almost the same anatomy and…

fermented 4a. Sucrose fermented…………………………………….Proteus vulgaris 4b. Sucrose not fermented………………………………Proteus mirabilis 2b. Sulfur not reduced. 5a. Lactose fermented. 6a. Sucrose fermented. 7a. Ornithine decarboxylase present……Enterobacter aerogenes 7b. Ornithine decarboxylase not present…….Klebsiella pneumonia 6b. Sucrose not fermented……………………………….Escherichia coli 5b. Lactose not fermented…………………………………….Serratia marcescens 1b. Glucose not fermented 8a. Glucose…

The purpose of performing physiological tests is to figure out certain characteristics of microorganisms or to reach a conclusion on what an unknown microorganism is. It can be also used to determine what is needed for bacteria to be cultured in or on certain media. Culture media can either be liquid or solid, and they can be used to identify certain characteristics of different bacterial species. This is indicated by the growth or lack of growth in or on certain media. Solid media can also be…

bacterial flora includes Staphylococcus aureus, Proteus vulgaris, Shigella species., Pseudomonas aeruginosa, Morganella morganii, Aeromonas hydrophila, Enterococcus species, Serratia marcescens, Proteus mirabilis, Shewanella putrefaciens, Enterobacter aerogenes, Salmonella typhi, Citrobacter freundii, Klebsiella pneumoniae, Bacillus subtilis, Bacillus megaterium, Bacillus cereus etc and anaerobes includes Clostridium perfringens, Streptococcus species, Bacteroides species(). R.S. Baylock 2001…

broth which was originally a purple colored medium turned into a color yellow and signs of gas production was also noted. The result indicated that the unknown #63 was positive for both acid and gas production and eliminated K. pneumoniae, and E. aerogenes because they can produce acid, but no gas production was suggested from the dichotomous key. The test result for SIM deep slant that was used to test for sulfur-reducing turned up positive due to its color changed into a blackening color. The…