Unknown #29 was Enterobacter aerogenes. But for good measure as a Final test I used the Methyl Red-Vogues Proskauer test to double check my results. The MR-VP test was performed, giving the expected negative result. This indicated that the unknown did not produce acids as a byproduct of glucose metabolism. The negative result eliminated K. pneumoniae from the list of remaining possibilities. The Voges-Proskauer test demonstrated a positive result indicating that Enterobacter aerogenes was…
catalase. Lastly, the oxidase test resulted in a negative reaction. Meaning that my unknown is not an oxidase-producing microbe. Using Appendix E of the General Microbiology Laboratory lab handbook, I narrowed down the unknown microorganism to Enterobacter aerogenes. Every test matched with my experimental results except for the fermentation of lactose. According to Appendix E, E.aerogenes is able to ferment lactose however my enterotube experiment yielded a negative result. I assume this…
Enterobacter spp. Enterobacter spp are part of the enterobacteriaceae family. They are facultative anaerobic gram negative bacilli. Their means of motility is by means of petrichous flagella. They produce acid upon glucose fermentation and are methyl red negative, voges-proskauer positive. Optimal growth temperature is 30*C .80% are encapsulated. E. spp have been associated with nosocomial outbreak and are opportunistic pathogens. They can cause numerous infections such as cerebral abscess,…
energy from both aerobic and anaerobic environments (Britannica). E. cloacae is a nosocomial pathogen, more commonly contracted in the ICU. Enterobacter are ubiquitous in nature. Animals contain Enterobacter in their intestinal tracts allowing for further transmission of the bacteria in soil, water and sewage (Britannica). In humans, pathogenic Enterobacter, such as E. cloacae, result in bacteremia, urinary tract infections (UTIs), intra-abdominal infections, endocarditis, lower respiratory…
From the patient, unknown bacteria #618 was identified to be Escherichia coli. It is apart of the Enterobacteriae genus that according to Brown’s Microbiological Applications1 are gram-negative bacteria that usually live inside the intestines of animals and humans. The organism can also live in the vagina and urethra. The data collected after isolating the organism and executing a series of tests have confirmed previous observations of E. coli in past scientific history. For the majority of…
lactose fermentation. The MacConkey test can be seen in figure 6, however, the results lactose fermentation results are not easily seen on the MacConkey’s plate. These two tests narrowed the bacteria down to three choices: Citrobacter freundii, Enterobacter aerogenes, and Escherichia…
cultures: Escherichia coli, Enterobacter aerogenes, Staphylococcus epidermidis, and Streptococcus faecalis showed observable growth when applied. The different medias will either promote or inhibit the growth of the organisms allowing their isolation to be possible (Lengeler and Schlegel, 1999). These four types of organisms will react accordingly based on their preferable environmental conditions. Observing their growth…
putida, Acinetobacter baylyi, Escherichia coli, and Enterobacter aerogenes by the streak-plate method, which can be seen in Table 2. As demonstrated in Table 2, none of the antibiotic producers were found to be resistant against Enterococcus faecalis or Pseudomonas putida. Table 2 also showed isolates 3 and 4 were resistant to Acinetobacter baylyi, isolate 12 was resistant to Escherichia coli, and isolates 12 and 16 were resistant to Enterobacter aerogenes.…
megaterium, S. aureus, S. epidermis, M. roseus, and M. luteus. Additionally, I was also able to reduce the possible bacteria for 7B to one of the gram negative bacteria. The gram negative bacteria included, P. vulgaris, E. coli, C. freundii, E. aerogenes, A. faecalis, and P.…
and biochemical testing were performed to identify the bacterial unknown for this project out of three possible options: Enterococcus faecalis, Enterobacter aerogenes, and Serratia marcescens. After careful…