Enterobacter aerogenes

the tube but stayed rather stationary and near the bottom, showed that it appeared to be anaerobic, but this conclusion is inaccurate with my hypothesis, since Enterobacter aerogenes is considered to be “facultative” based on page 85 of the lab manual appendix E (MB 352 Laboratory manual pg 37, 41, 85). Additional testing was done in lab 5, from which we utilized tests with “SIM agar deep tubes, nitrate reductase, protein hydrolysis, catalase, and cytochrome oxidase” (MB 352 Laboratory manual pg 48). The “SIM test” or “sulfide-Indole-Motility” had three parts to see “sulfide production”,…

microorganism to be Enterobacter Aerogenes. The plan to identify the microorganism was to grow a pure and uncontaminated…

E. aerogenes has a BSL level of 1 but can cause urinary tract infections and bacteremia (Dworkin et al., 2006). It is a motile bacteria that can move around if media allows (Smalley and Kaltz, 2003). The typical appearance of E. aerogenes on complex media is round medium colonies that are slightly raised or flat with irregular margins (Dworkin et al., 2006). On MacConkey’s agar, E. aerogenes will grow, as it is gram negative, and will appear pink as it can metabolize lactose; in blood agar it…

Unknown Bacteria 228 Adisney Pino Professor Dowding Miami Dade College Section T/R 7:05PM Introduction Enterobacter aerogenes is rod-shaped bacteria that belongs in the family of Enterobacteriaceae. This rod-shaped bacterium is gram negative which makes it a facultative anaerobe which grew perfectly in 37-degree temperature. This species was motile and therefore it can synthesize an enzyme known as ornithine decarboxylase. This bacterium is prevalent in the intestines of…

1) Alcaligenes faecalis is a Gram-negative coccobacilli that is motile, oxidase positive, catalase positive, non-spore-forming, non-fermenting, slow growing, and citrate positive obligate aerobe (Mahon et al., 2014) (Sachdeva et al., 1963). Its colony morphology is flat, thin, and rough with irregular edging (Mahon et al., 2014) and the flagella varies between the usual shape versus the curly structure, however, both shapes do not exist on the same individual bacteria (Leifson, 1960). The…

antibiotics, or improper use. By determining the resistance and cross-resistance, one may determine the the relative ability for bacteria to gain resistance to antibiotics. We hypothesis that if we expose Enterobacter cloacae to Tetracycline over a week, then the bacteria will evolve a resistance and the zone of inhibition will decrease. Also, the bacteria will gain a resistance to other antibiotics and biocides, such as Triclosan.…

Introduction Identifying microbes are important for working in the medical fields and also for research. Physical and cellular processes are ways in which microbes can be identified. A series of test were done to identify an unknown microbe labeled 5. Based on observation and the results conclusion was made to identify the bacteria as Enterobacter aerogenes. In microbiology, researchers are frequently trying to culture and classify microorganisms in a laboratory setting for study. Because of…

The symptoms reported were vomiting, bloody diarrhea, and severe cramping which is a gastrointestinal infection. It was also mentioned that raw bean sprouts were possibly the culprit for this and people were warned not to consume them. Although it can cause GI distress in some as mentioned earlier, Enterobacter aerogenes is a hospital acquired infection. This would mean that all the cases that were reported would have had to have been associated with a recent hospital stay. The main ways to…

E. aerogenes is a non-spore forming organism with 1.2-3 μm in length by 0.6-1 μm in diameter and belong to the Enterobacteriaceae Family. E. aerogenes have capsule with flagella which enable them to be motile. They are facultative anaerobes which mean they are able to survive with or without the presence of oxygen. The optimum growth temperature is range from 30oC to 37oC which classify as a mesophile. It is commonly associated E. aerogenes with nosocomial infection and they are acknowledged as…

microscope, and observe the slides under 10x and 100x objectives. Draw and make observations over what is seen, take note which dye produces a clearer and more defined bacterial cell. 10. After observing the bacteria under a microscope and determining which dye creates a better looking bacterial cell image, stain the 3rd bacterial mount with that dye, following the procedure in step 8. 11. Observe the 3rd slide under the microscope using the 10x and 100x objectives, draw and make observations…