ELISA

flasks in a density of 40 million cells per 20 ml HMDM per flask. Phytohemagglutinin (PHA-M) were added in the medium to a final concentration of 5 μg/mL to activate the PBMCs. After activation, 1 ng HIV-1 p24/ml HIV-1Ba-L virus, measured by HIV-1 p24 ELISA (ABL inc., Rockville, MD), will be added to the culture medium to infect the PBMCs for 3…

Objectives: In the present study we made an attempt to evaluate the association of Plasminogen Activator Inhibitor-1(PAI-1)4G/5G polymorphism with oxidative stress markers Malondialdehyde (MDA) and High sensitivity C-reactive protein (hs-CRP), fibrinolysis marker PAI-1and lipid profiles. Subject and Methods: Blood was drawn and DNA extracted from 90 subjects (46 cases and 44 controls). The 4G/5G polymorphism of PAI-1 was amplified using specific primers. Amplified products were visualized by…

Mary Catherine is just one example, of the many children who have faced the consequences that come with not being vaccinated. Mary’s parents, Suzanne and Leonard Walthers, decided to research the safeness of vaccines for their new baby and found information that initially made them decide against getting Mary vaccinated. It is because of this decision, that Mary came down with a form of meningitis, one that had nearly been eradicated in the U.S. and could have easily been prevented with a…

Throughout the centuries knowledge can be in various ways be claimed to be the key component to developing a strong civilized society. This can be demonstrated in the novel Lord of the Flies, throughout the novel, this certain concept of knowledge is present, especially when the group of boys decide to use perhaps one of the most symbolic objects in the book, the specs that belonged to Piggy to create a fire that was used to signal any kind of ship or plane. “‘Piggy! Have you got any matches?’ ……

B. Introduction/Background/History: Ebola is a virus that is very deadly. Four out of five strains can make people sick. While inside of the body it is possible to do the following things: kills cells, full bleeding internally, wrecks the immune system, and harms every organ in the body. It is very infrequent to catch Ebola. The only way that people can get infected with the disease is from direct contact with fluids from those who are already infested with Ebola. C. Content: Ebola was first…

How Social Media Impacts the News We live in a truly wonderful time. The internet has connected our world in an unprecedented manner. Everyone with internet access has the entirety of human knowledge at their disposal and a means to contact nearly anyone in the world instantaneously. A product of the internet is what we refer to as “social media.” Social media makes it extremely easy to keep in touch with friends, family, and practically the whole world. Facebook, Twitter, Snapchat, and a…

While the costs for this study are considerable and the outcome could have significant impacts on how we view the relationship between health, exercise and eating habits, it is entirely unacceptable for Dr. Powell and the Center to accept sponsorship money from Kiddie Kola and Burger Shack. This sponsorship money will undermine the objectivity of the research and the legitimacy of the results. It should go without saying that the only reason Kiddie Kola and Burger Shack are willing to cover…

Why It's Real A recent study revealed that patients with chronic fatigue syndrome have low levels of cytokines in their blood, especially in the early stages of the illness. Cytokines are proteins made by the cells of the immune system. They are responsible for blocking viral replication and driving inflammatory response. Where You Can Do More Research http://www.psmag.com/health-and-behavior/clues-the-immune-system-is-behind-chronic-fatigue-syndrome http://www.washingtonpost.com/news/to-y…

The Elon Journal of Undergraduate Research in Communications 4 (2013): n. pg. Web. 28 Nov. 2014. "Every Girl - Lil Wayne." SongLyrics.com. N.p., n.d. Web. 16 Dec. 2014. "Hurt Me Soul" Lyrics." LUPE FIASCO LYRICS. N.p., n.d. Web. 20 Nov. 2014. Puvia, Elisa, and Jeroen Vaes. "Being a Body: Women's Appearance Related Self-Views and Their Dehumanization of Sexually Objectified Female Targets." Sex Roles 68.7-8 (2013): 484-95. ProQuest. Web. 14 Nov. 2014. Rhym, Darren. ""Here’s for the Bitches": An…

The genre of the both plays were comedy as they both treated their respected subject matter with humor. The first play ‘The Pretentious Young Ladies’ dealt with the theme of pretentious nature of people, while the second play, ‘The Miser’, dealt with the theme of greediness that plagues many human minds. Considering the sound of laughter abundant among the audience on that day, I consider both plays successful in gaining the response they both aspired to. In the first play, ‘The Pretentious…