ELISA

Did you know thinking of yourself as fit actually makes you fitter? That's according to a 2015 study conducted at Texas Woman’s University. Researchers looked at a group of healthy women aged 18-30 and found that those who perceived themselves as strong and fit scored significantly higher on fitness tests than women who had lower self-esteem. The women were otherwise similar in BMI and frequency of exercise, which supports that there is in fact a psychological component to fitness. “If you…

It has been demonstrated that MUC1 (membrane bound mucin) often located on the outer portions of cells, serves as a preventive agent against Helicobacter pylori. MUC1 reduces gastric inflammation in H. pylori Infected tissue by forming a complex with IkB kinase gamma (IKKγ) in this way hindering the contact between IKKγ and IKKβ causing the prevention of nuclear factor kappa B (NF-kB) transportation to the nucleus, in this manner preventing the transcription of genes that can cause inflammation…

Ebola virus disease EVD or “Ebola” is a severe human and non-human primate disease caused by Ebola viruses. These viruses belongs to the family: Filoviridae, Genus: Ebolavirus, Species: Tai forest ebolavirus (TAFV), Reston ebolavirus (RESTV), Sudan ebolavirus (SUDV), Zaire ebolavirus (EBOV) and Bundibugy ebolavirus (BOBV) (Bukreyev et al., 2014). Zaire ebolavirus or simply Ebola virus (EBOV) is the most virulent of the genus and accounts for the highest numbers of outbreaks. The virus spreads by…

Introduction Sold commercially under the tradename Sevin, carbaryl (C12H11NO2) is an insecticide that is commonly applied to corn, soybean, and cotton (EPA, 2000). It is a cholinesterase inhibitor that kills insects by preventing the breakdown of acetylcholine, a neurotransmitter. Neurons are stimulated when they release acetylcholine and transmit a message to the next neuron (Waymire, n.d.). However, in order for a neuron to return to its initial, unstimulated state, the acetylcholine in its…

Blood samples were taken before castration and at 12, 24, 48, and 72 hours after. In both experiments, behavior was observed and recorded by the same-trained person. The blood samples were analyzed for pro-inflammatory cytokines TNF-α and IL-1β by ELISA. No differences were found between experiments. In experiment one, castrated piglets had less movement behavior and stayed closer to the…

detection protocol used, should be completed under BSL3 conditions. For non-inactivated samples, RT PCR and enzyme-linked immunosorbent assay testing can be performed at a BSL3 laboratory. If samples have been inactivated (i.e. cell lysis) RT PCR and ELISA testing can be performed at a BSL2 laboratory. Laboratory diagnosis: Laboratory results should…

test the concentration of the various proteins involved in the transduction pathway. Each protein plays a major role, but since cAMP is the most relative, the detection of that protein will be discussed. The levels of cAMP can be detected using an ELISA kit. “cAMP levels are based on non-affinity-purified polyclonal anti-cAMP antibodies.” (4838136) To better visualize the levels of cAMP under a microscope, fluorescent manipulation was integrated into the process. This lead to accurate detection…

5. Dwivedi, et al., (2008) described that the Synthesis of 11-substituted estradiol derivatives (12–17) has been approved out by the Grignard reaction with alkyl, allyl, and benzyl halides on 17b-hydroxy-3-methoxy-11-oxo-estra-1,3,5(10),8(9)-tetraene (10). The novel compounds (10 and 12–17) were appraised for their preliminary post-coital contraceptive (anti-implantation) activity in Sprague–Dawley rats. The tested compounds were managed orally and exhibited noteworthy anti-implantation activity…

Figure 3.1.2. Antibody titres of rabbit antisera. The antisera were collected from immunized rabbits and antibody titres measured by indirect ELISA as described under Methods (section 2.2.3.1). 3.1.2.1.3 Purification of α-LA, HRP and human erythrocyte specific polyclonal IgG The IgG fractions were purified from rabbit sera by ammonium sulphate precipitation followed by negative ion exchange chromatography employing DEAE-cellulose. The antisera were decomplemented, IgG fraction precipitated by…

The ARA San Juan, which was last contacted off the coast of Argentina on November 15, has enough air to last only seven to 10 days if it has remained fully immersed since that time, experts say. If the submarine has surfaced or "snorkeled" -- that is, raised a tube to the surface to refresh the vessel's air -- since then, the crew may have bought more time. Anxious families have been waiting at Argentina's Mar del Plata navy base, to which the submarine was heading when it vanished, for news of…