DNA

Objective: We started by isolating a DNA plasmid from a bacterial culture for cloning. Then, we reverse transcribed mRNA to amplify it in a polymerase chain reaction. Finally, we amplified a specific gene (actin) through PCR. Protocol: A. Isolation of Plasmid DNA from a Bacterial Culture: We were provided with a tube containing 1 ml of bacterial culture. We centrifuged it for 5 minutes at 10000 g. Then, we removed the supernatant by pouring it off and we used a small chem wipe to remove any…

What aspects of DNA’s structure contribute to the stability of the DNA molecule? The DNA’s double helix structure and the hydrogen bonding that bonds the bases contributes to the stability of the DNA molecule. 2. The DNA sequence is: 5’-ATTCGACC-3’. What will be the sequence of the complementary strand using this strand as the template? The complementary strand would be TAAGCTGG. 3. When the base composition of double-stranded DNA from a new species of bacteria was determined, 30 percent of the…

our Human DNA is junk or not. Although valid points are made from both sides, throughout the article there was a tendency where scientists agreed at some degree that some of the non-coding DNA is junk. Some of the views that the article focuses on are aligned in broad spectra of agreement whether or not most of our DNA is complete garbage. Scientists like Ryan Gregory agreed that complexity of an organism is irrelevant to its genome, because most of DNA (about 98.8 percent) is noncoding DNA,…

structure dictates its function in storing the genetic information of the organism (Rafael, 2010). This molecule contains genes which encode proteins needed for the complex biochemical metabolic reactions which occur within the organism (Rafael, 2010). The DNA molecule consists of 2 complementary strands in a helical structure where each strand serves as an informational template for the offspring during duplication (Rafael, 2010). An organism is made up of a vast collection of cells where each…

Assignment 3.05 DNA DNA, deoxyribonucleic acid, is the stuff in humans and almost all other organisms. About every cell in you and everyone else's body has the same DNA. DNA is usually located in the cell nucleus (nuclear DNA),however small amounts of DNA can be found in the mitochondria (mitochondrial DNA or mtDNA). The DNA has information stored as a code created by four chemical bases: adenine (A), cytosine (C), guanine (G), and thymine (T). Each base is connected to a sugar molecule and…

I. Summary I read the NYT articles called: DNA and Mutations, Down syndrome, and Ethics Questions Arise as Genetic Testing of Embryos Increases. To summarize all of the readings, I believe it would be essential to start with the DNA and Mutations article. In the article DNA and Mutations, scientists have found two different genetic occurrences in multiple individuals bodies called, mosaicism and chimerism. Chimerism involves a single organism who contains genetically distinct cells, which most…

The National Institute of Justice’s article “Extending Time to Collect DNA in Sexual Assault Cases” explains the usage of DNA profiling in identifying a suspect in a sexual assault case, but the current standard DNA profiling methods have different shortcomings. DNA profiles often require the use of short sequences that repeat a number of times, called short tandem repeats (STRs). With the current DNA profiling method, many jurisdictions require the samples to be collected within three days of…

States should implement a DNA Database for all criminal suspects. According to criminal.findlaw.com, a DNA database is a national data system filled with DNA profiles. It is usedfor many different reasons, but today we are focusing on it helping to solve crimes all around the country. While this idea sounds picture perfect, there are many issues that are failed to be mentioned when this topic is brought up. My team and I are here today to express the dangers of a national DNA Database. My…

of extraction of plasmid DNA, which we used Resuspension Buffer to break up our pellet after removing the supernatant (liquid) after centrifuging our overnight culture. Then Lysis Buffer was used to break down the membrane of the cells so that we could then add Neutralization Buffer to bring our sample from basic to neutral pH. After centrifuging a few times with some steps in between we added Wash Buffer to remove all other liquid from the spin column leaving just the DNA in its membrane. Later…

DNA in Animal Forensics I love watching murder mysteries shows on TV. In a lot of the shows suspects are caught by DNA evidence, but this has not always been the case. DNA study has progressed a lot over the recent years. One of the areas it has progressed, is using DNA in animal forensics. DNA profiling has changed a lot since it first become popular back in the 1980’s. Although using animal DNA to convict a suspect was not always accepted it has since been accepted to peer review journals.…