DNA repair

Firstname Lastname Instructor’s Name Course Number Date Genetically modified canola: Agronomic benefits and environmental disadvantages The world’s food source is facing selection and sustainability pressures from unpredictable climatic changes, massive growth in population and continuous reduction in agricultural land (Nicolia et al. 77). Genetically modified (GM) crops seem to be the solution to feeding billions of people, although their negative long-term impacts are still a heavily debated…

identify genes that gives rise to phenotypic effects when they are switched off, and also helps to analyze the effects of these genes in cells or complete organisms. The first step in the gene trap process is to integrate an artificially constricted DNA into a genome of a cell line, usually an embryonic stem (ES) cell line. To carry out this…

Plasminogen Activator Inhibitor-1(PAI-1)4G/5G polymorphism with oxidative stress markers Malondialdehyde (MDA) and High sensitivity C-reactive protein (hs-CRP), fibrinolysis marker PAI-1and lipid profiles. Subject and Methods: Blood was drawn and DNA extracted from 90 subjects (46 cases and 44 controls). The 4G/5G polymorphism of PAI-1 was amplified using specific primers. Amplified products were visualized by staining with Ethidium bromide after electrophoresis in agarose. Risk factors PAI-1,…

Chemistry: Enasidenib is a first in class, a selective allosteric inhibitor of mutant isocitrate dehydrogenase-2 (IDH2) inhibitor discovered at Agios Pharmaceutical, Inc. in the United States of America. Recently, on 1 August 2017, Celgene Corporation and Agios Pharmaceutical in cooperation won the approval for Enasidenib under brand name IdhifaTm for the treatment of relapsed/refractory acute myeloid leukemia. it is a white to off-white crystalline, non hygroscopic powder existing in…

The fungi Alternaria is a phyto-pathogen cause leaf blight disease to variety of plants. Alternaria alternata and Alternaria helianthi was isolated from Dalbergia sissoo L. and Helianthus annuus L. respectively. Test antagonists grow faster than the pathogen and produced inhibition zones thereby limiting the growth of the pathogen. These antagonistic interactions influence the incidence and severity of the disease caused by the pathogen. When the two Alternaria spp. were tested by dual culture…

Wide hybridization and characterization of hybrids of Linum usitatissimum L. for crossability, various agro-morphological traits and rust resistance Nimit Kumar*, Satish Paul, H.K. Chaudhary, Navdeep S. Jamwal and Amaninder Deep Singh Department of Crop Improvement, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, India For correspondence: nk.kakran@gmail.com SHORT SUMMARY: The present research has yielded a wide array of germplasm which will serve as a potential source of…

transfers the genetic code from DNA via mRNA to finally form the functional product (finished proteins) together forms the central dogma of modern molecular biology. Thus, the genetic code is the basis of the central dogma of molecular biology. Central dogma is nothing but the flow of genetic information in all living cells including human cells from DNA to RNA to proteins. The central dogma There are three classes of sequential biopolymers that encode information: DNA, RNA, and protein. The…

Molds are multi- or unicellular while yeasts are unicellular in structure. Molds grow in the form of tangled mass called the mycelium which is composed of filamentous hyphae while yeasts are spherical or oval-shaped. Molds are colourful while yeast are colourless. Molds can reproduce through small spores sexually or asexually while yeast reproduce asexually by budding or binary fission through mitosis in which a nucleus is divided into two or more nuclei. Mould can grow on food with low pH…

Km and Vmax determination of glucocerebrosidase towards p-NPG The Michaelis constant (Km) is a measure of the affinity of the enzyme towards the substrate, with smaller values representing greater affinity. Km and the maximum rate (Vmax) of leukocyte GCase were obtained through the Lineweaver-Burk plot (Figure 4) with artificial substrate p-NPG in concentrations from 0.71 mM to2.50 mM. Km and Vmax values for leukocyte GCase using p-NPG as substrate was found to be 12.6 mM and 333 U/mg…

Forensic science is an application of all types of science field, especially chemistry to help with the investigation that related to law or crime. It is approved aspect to be applied in a court or law. In this field, forensic scientist plays an important role to help in the investigation. The function of forensic scientist can be classified into three parts that are: 1. As a crime scene investigator As a crime scene investigator (CSI), a forensic scientist involve in collecting the evidences.…