Dish Network

A. Description of the Study Area The experimentation of the study will be conducted in PLM College of Science Laboratory at Pamantasan ng Lungsod ng Maynila, Gusaling Lacson, 6th floor. The University is located beside Department of Labor and Employment building in Intramuros, Manila, known as the “Walled City”. At the back of the University is the Philippine Red Cross - Manila Chapter. The Lacson Building where the laboratory is located is in between the University Gymnasium and Justo Albert…

bacteria before conducting the environmental sampling lab. I knew that bacteria could be found in many different places. Also, different types of bacteria grow in different conditions. From prior experience, I remembered that to grow bacteria in a petri dish one must follow the proper technique. I chose to test how much bacteria grows on toilet handle in the bathroom. My prediction for the experiment before it was conducted was that there would be a large amount of bacterial growth on the…

pot, with water in it to be a boil. Water level needs to be right under the label on the agar bottle, with the cover losing, or it will explode. Agar needs to be boil for about 30 to 45 minutes, until agar liquefied. Then pour agar inside the petri dish, about half full. Make sure agar is fully liquefied, or it will have bubble spots in agar. Make sure all four Petri dishes are half full. Seat agar plates out for room temperature for about one to two hours, end then place them in the…

The Effect of Carbon Sources on Yeast Growth Introduction Biofilms are encountered every day through a multitude of ways whether it’s by dental plaque on our teeth, in our showers or on medical equipment. (Hall-Stoodley 2004). A biofilm is formed when single bacterial cells stick to each other (Schussler 2014). Biofilms as a group converse and work together as a complex unit which allow them to be effective in growing, surviving and reproducing (Liu et al. 2015). Moreover, yeast biofilms are an…

Oral Microbiome 10: Reflection 3 One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…

Objective I. To learn how to inoculate the nutrient broth properly in preventing contamination to the nutrient broth and the culture Escherichia Coli. This is done by sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on. II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”. III. To learn how to do a complex streak where we grow E. coli culture on the nutrient…

Vocabulary 1. Inoculum: a material used for inoculation. 2. Loop (the tool): A wire made into a loop to assist in picking up inoculum from a culture of microorganisms. The loop is also used in the process of cultivating microbes by transferring inoculum for streaking. 3. Agar vs Blood Agar: a. Agar: a growth medium made with agar and other nutrients. The agar allows microorganisms such as fungi and bacteria to be cultured. b. Blood Agar: Blood agar is used for the purpose of cultivation and…

Procedure 1. Find the materials. 2. Take your four postit notes and label them tap water, well water, spring water, or distilled water. 3. place the Post-it notes on a flat surface. 4. Fill each jar with one cup of one type of water. 5. Place the jars on top of the correct Post-it note. 6. Take one small piece of duct tape and place the top line of the tape at the top water line for each jar. 7. Make a table like the one shown. 8. Put however much soap you want in A soap container. 9. Bill the…

In order to obtain accurate numbers from the now grown-out Sordaria samples, a small procedure was followed. First, a sample of fungi was taken from the border of a WT and TT section in the Petri dish. A slide was prepared by placing one drop of water on the middle of it, the Sordaria sample on top, and a slide coverslip on top of the sample. The eraser of a pencil was used to lightly press on the coverslip in order to release the asci so that they…

twice using the p1000. Both tubes were then centrifuged for 1 minute to separate the pellet and the supernatant. The supernatant, 1 mL from each tube (2 mL in total), was then filtered through a .22 micron filter in order to retrieve phages. A petri dish was divided in four sections and labeled as positive control,…