Dilution

Abstract The main goal of the buffer capacity experiment was to analyze how the addition of acid or base and dilution affected the measured pH. The results showed that when the buffer systems were under acidic conditions their pH values decreased and increased when exposed to a basic environment. The main goal of the ionization of the amino acid experiment was to titrate an unknown amino acid with a strong base in order to produce a titration curve. The titration curve was used to evaluate the…

Solution CTQ 04: Fundamental Concept:  An agent lethal to bacteria is bactericidal and which inhibits the growth of bacteria is bacteriostatic.  Effectiveness of control agent depends on its concentration, time of exposure and its activity (cidal or static) Solution: • Microbial control agents are of two types based on their effect on microbial cells which are either bactericidal or bacteriostatic. • Bactericidal antibiotics kill (cidal) the bacteria Ex: Polymixin B is a bactericidal agents…

bacteria with new DNA so that is expresses different genetic information. This is done by affecting the bacteria transformation of E coli bacteria by using heat shock and incubation. The hypothesis for this study is that the one with no antibiotic or dilution there would be tremendous amount of E. Coli bacteria and with antibiotic (ampicillin) the amount of E. Coli will be lower however if the E. Coli is diluted the amount of bacteria count will be lower because the E. Coli is resistant to…

Increasing level of education has added to the dilution. Increasing education had so far meant better literacy and incidental knowledge that accrues, which raises awareness of rights, instigates cutting corners to extract advantage, avoiding hardships and avoidance of responsibility and obligation, that…

The dilutent was distilled water. The BSA was placed on the ice prior to being mixed into the four micro-tubes mentioned before. The 20ml dilution was added into the four micro-centrifuge tubes including the control. Two additional tubes were added, one with 20ml of distilled water and the other was unknown. After that, 980 micro liter diluted Bradford dye was added into the four microtubes…

mentioned to approximately measure certain amounts of reactant to later solve for initial concentration the graduated cylinder was not the most reliable instrument to measure the volume of liquids accurately. It was also important to note that in a dilution calculation, concentration and volume are inversely proportional to one another. If there’s an increase in volume, then there is a decrease in concentration, vice…

Abstract This experiment observed the effects of enzyme’s concentration on the enzyme’s activity. This was determined by measuring the rate of the reaction under different concentration, and see whether the reaction increases when the concentration high or not. The consequences of the experiment indicated that the rate of the reaction of enzyme increases whenever the concentration is high. Also, if the concentration is low, the reaction rate will be low too. Introduction Enzymes are…

though c3 one at a time though the spectrometer. Record each data. Then repeat the process for E1 though E3. Record the data also. If the spectrometer cannot read your control or experiment tubes then a dilution factor must be used. In this experiment the spectrometer could read all six test so no dilution factor…

Whether it be the gasoline our cars utilize or the electrical currents that power our phone, everyone uses energy in some form. Most of the time, the energy being used are fossil fuels, e.g., crude oil, natural gas, and coal. Despite the wide audience that reap the benefits that fossil fuels provide, they actually do more harm to the Earth than most people think. 85% of America’s fuel usage comes from fossil fuels. The usage of fossil fuels do provide immense amounts of energy throughout…

Sterile Saline (0.9% w/v NaCl) Dissolve 9.0gm of NaCl in 1000ml water and dispense 9.0ml of the solution into 20ml test tubes. Autoclave the tubes as per the validated cycle and cool to room temperature. Sterile saline with polysorbate 80: Dissolve 9.0gm of NaCl in 1000ml water and add .5gm of tween 80 to the resulting saline solution, dispense 9.0 ml of the solution into a number of 20ml test tubes. Autoclave the tubes Preparation of Inoculums and its Standardization: 1) Take loop full of…