Dilution

One German shepherd, nine greyhounds, and one pug were dropped from the acquisition study due to motivation failure. The study continued with dilution training, which tested the dog’s performance when the odorant was diluted. All of the dogs from acquisition training participated in dilution training. The full strength odorant was one milliliter of the anise extract, the 10% odorant was made from one milliliter of anise extract and nine milliliters of mineral oil…

Spectrophotometry is a method in which organic and inorganic substances are identified or characterized by the use of electromagnetic radiation. Electromagnetic radiation not only includes light (or photons), but thermal energy as well. While the electromagnetic spectrum consists of wavelengths between gamma radiation and the Far IR, most spectrophotometric methods rely on the UV and visible region. In order to use spectrophotometric methods, the sample being analyzed must be able to absorb the…

(MP Biomedicals), 10mM MgCl2, 1.5mM FBP (Sigma Aldrich), and 0.2mg/mL Bovine Serum Albumin (Sigma Aldrich), and 1.5mU/µL yeast inorganic pyrophosphatase. ATP was added, whose final concentration was 1.5mM and the rest of the volume was the desired dilution of the protein solution in question, water, or pyrophosphate (Sigma Aldrich). Assays were initiated with ATP or water and incubated for 10 minutes…

Introduction: Amongst the Listeria species, only Listeria monocytogenes is considered to be a significant human and animal pathogen, and is distinguished by its ability to cause hemolytic activity, which is usually tested with conventional sheep blood agar plates (Perrin and others 2003). This indicates a risk where they are present in food products, and are more prevalent in ready-to-eat (RTE) meat and poultry products. Being ubiquitous in the environment and in food, Listeria innocua is…

A series of dilutions were prepared by mixing the stock solution was water in a test tube. The concentrations used were 0.139 mg/mL, 0.111 mg/mL, 0.083mg/mL, 0.057mg/mL and 0.028 mg/mL. There were two lots of each concentration made. The spectrophotometer was set at the…

repeated aseptically, transferring 10 microliters of solution from one tube to its successive tube in order to dilute the phage. The 5 tubes were then used to infect 5 different tubes of Mycobacterium smegmatis. These infections were plated. This dilution series was repeated 3…

this second round of purification, I picked a relatively isolated plaque and used that to do a serial dilution, from this dilution I could do my first spot titer test. This test would allow me to collect my small volume lysate, and calculate the concentration of my small volume lysate which happened to be roughly 3.3*10^10. This spot titer test gave me the opportunity to calculate what dilution I should do in order to consistently make webbed plates. I made my first webbed plates and flooded…

devided to three main groups: Can type, Can Annular type, and Annular type chambers. Combustion chambers consist of components such as swirler, injector, diffuser, liner, cooling holes, dilution holes and etc. A gas turbine combustion chamber classically has three zones: primary zone, secondary zone, and dilution zone. In primary zone, which is located between injector and primary jets,…

(Passage #1)The Dilution of Reasoning: An Analysis of the Issue of “Impressions” and “Ideas” in Hume’s Analysis of Human Thought This philosophical analysis will define the premise of impressions and ideas in Hume’s rejection of cause and effect as a form of human reasoning. In this perception, Hume is defining the vivid nature of Impressions, which eventually become diluted into ideas over time. Hume’s analysis of this phenomenon reverts to the initial premise that cause and effect cannot be…

Introduction The cultivation and enumeration of a bacteriophage is a vital component of microbiology. Bacteriophages are used for a number of scientific applications, including the decontamination of food products, human therapy, and much more, thus it is critical to determine the concentration of the bacteriophage in a solution. Doing so allows scientists to determine bacteriophage potency, side effects, and minimal effective doses for clinical applications (Andersen et al., 2011). This…