Dilution

The molar mass of KHP was 204.22g. Molarity x Volume (L) = Moles of KHP Moles of KHP x Molar Mass of KHP = grams of KHP 0.1 x 0.05 = 0.005 moles of KHP 0.005 x 204.22 = 1.0211 g KHP Dilution of NaOH and HCl To find out how to dilute NaOH and HCl the equation M1V1 = M2V2 is used. For both the M1 and V1 were given as 0.1 and 250 mL respectively. The M2 was given as 3. The equations were set up as follows: g HCL = 0.1 x 250 = 3 x V2 → (0…

20 μg of mitochondrial fraction were applied for each reaction, and for each trial duplicate reactions were done. In order to evaluate total mitochondrial cytochrome-c oxidase activity, the isolated mitochondrial fraction was diluted in the enzyme dilution buffer (10 mM Tris–HCl, pH=7.0, containing 250 mM sucrose) with n-dodecyl β-D-maltoside (1 mM), and then put it on ice for 30 min. The reaction was started by adding ferrocytochrome-c substrate solution (0.22 mM) to the mitochondrial sample.…

Purifying DNA to Estimate its Purity using PCR Amplification of VNTR to Load and Run Agarose Gel Introduction The study of this experiment was the Dopamine transporter gene. This gene is associated with different brain disorders like bipolar, as well as certain behavioural traits such as ADHD.[1] Dopamine transporter gene is a presynaptic plasma protein containing different VNTRs in it’s UTR and plays an important role in restricting the activity of dopamine by rapid reuptake into the…

FirstBank is fortunate because the top four banks in its market are not local community banks. This gives the Bank an advantage in local relationship-based banking and customer loyalty for being a local business. The same customer base also relies on a local bank to provide quality products and services like the big national banks. FirstBank stays on the leading edge of technology which helps portray a big bank environment and provides all the products and services offered by the big banks.…

Purification and Quantitation of DNA and Plasmid Structure Labs 3 and 4 Biology 453 Clayton Pedigo September 14 and 21, 2016 Introduction: In this report, there are two labs being discussed. The first lab being lab 3 (purification and quantitation of plasmid DNA) involved lysing bacterial E.coli cells and retrieving the plasmid particles. The purpose of the second lab, lab 4 (plasmid DNA structure), was to use the concentrated DNA from lab 3 and compare…

Leptospirosis is a zoonotic disease, caused by the bacteria genus Leptospira, that can cause a variety of symptoms and which, untreated, may lead to meningitis, kidney damage, or even death (“Leptospirosis”). Leptospires, bacteria of the genus Leptospira, appear as tightly coiled spirals and achieve optimal growth at 28-30°C, thriving in warm and humid regions. However, because most tropical, humid countries are also developing counties, chances of exposure are greater in those areas (Levett).…

1. Brief history of the company/factory ? In 1930, Dr. Minoru Shirota, who was conducting research in a microbiology lab at the Medical Faculty of Kyoto University in Japan. He succeed in culturing a strain of lactic acid bacteria beneficial to human health. This bacterium was named "Lactobacillus casei Shirota strain. It took Dr. Shirota another 5 years of research to find the right food medium for the Lactobacillus casei Shirota bacteria. In 1935 the first bottle of Yakult, a fermented…

Wouldn’t it be great if we could make the best cold brew in town without leaving the house? Want to know more about what it takes to create your own batch, perfected to your personal liking? Think it’s impossible? Well, think again. It’s easier than we can imagine. In fact, part of the joy of brewing is learning the best coffee for cold brew. It's fun to discover and create favorite recipes. All it takes is a bit of patience and a lot of enthusiasm. Are you ready to learn everything you…

3.1. Chemical composition and monosaccharide analysis Polysaccharide molecules are linked together by water molecules through hydrogen bonds in an aqueous solution. Adding ethanol into aqueous solution of polysaccharide initially dehydrate polysaccharides, enhance intra-molecular hydrogen bonding, cause conformational change and aggregation of polysaccharide molecules, and eventually leads to the precipitation of macromolecular chains, and the following addition of alcohol solvent gradually…

While complications also arise from the genetically manufactured food sources, it is the Clones who are relied on as symbols of future perfection. In order to prevent dilution of the perfect society represented by the Clones, homosexuality is trained into society to prevent the birth of non-Clone children. Although a number of individuals, called “deviants”, still maintain heterosexual relationships and engage in biological…