Dilution

Tests 1. Dilution Method Broth Dilution: this method involves exposing isolates, to a series of concentrations of antimicrobial agents in a broth environment. For this method, the lowest concentration at which the isolate is completely inhibited is recorded as the minimal inhibitory concentration (MIC). The test is only valid if the positive control shows growth, and the negative control shows no growth. Agar Dilution: this method has the same procedure as the broth dilution. Agar dilution…

Mutagenic Capabilities of Ethyl-Methanesulfonate Biology Lab 10501 November 30th, 2016 Lab Group: Drew Garza, Brock Morgan, Christian Chen, Daniel Lee. ABSTRACT INTRODUCTION Mutations are permanent changes in DNA sequence that makes up a certain gene that are caused by environmental factors or when DNA is copied in the replication phase. Mutations can range in various sizes and can affect any part of the DNA sequence. In our lab we dealt with Ethyl…

(dopa) to dopaquinone quickly converted to dopachrome in the presence of oxygen. It is interesting how the direct product of dopa, dopaquinone was never really measured and neither was PPO extract enzyme. Furthermore, the differences between the dilution factors 1/5 to 1/10 was a factor of ½ similarity to 1/20 and 1/40 as displayed on the graphs. This might be due to collisions between the molecules. Since temperature plays a huge role by affecting the shape and activity of enzyme, it must be…

The purpose of this experiment was to analyze meso- and microzooplankton feeding. Largely due to size differences, mesozooplankton (>140 μm) are important exporters of carbon and prey for larger organisms, while microzooplankton (100–140 μn) are important recyclers of nutrients, dominant grazers of phytoplankton, and key link in the microbial loop (Conroy et al., 2016; Uitto and Hallfors, 1996). Therefore, understanding this relationship between zooplankton and grazing will allow scientists…

Aileen San BIO 210L – Section 4 A Prokaryotic System for Transformation, Expression, and Purification of Eukaryotic Proteins Introduction: The bacteria E.coli is found in many places such as in animal intestines and the environment. These bacteria have a simple structure and are quick to reproduce (Centers for Disease Control and Prevention, 2015). Because of this, scientists know a lot about E. coli. In this experiment, the E. coli will be exposed to a pGLO plasmid; each plasmid has an…

1. The Case Holyday Inn is a chain of medium class three+ star hotels aiming at families and tourists. They have a registered brand and have the logo in green written in specific style. They have a competitor that is called Haliday Inn – two stars, aiming at low class tourists, backpackers, etc. They use different color (blue) and style. They position themselves as a hostel, make advertisements on the internet and have no registered trademark. 2. Things to consider before making the decision…

dense foliage and animal traffic near a large pond. These samples were tested using nutrient agar for “simple” soil bacteria, glycerol yeast extract agar for Actinobacteria, and Sabouraud dextrose agar with antibiotic for fungi. For the first step, dilutions were prepared.…

the eggs were bad and we would not get any plutei larvae from this batch. Table 1: Gamete Sizes: Difference in egg and sperm size. Egg size .05 mm Sperm Size .001 mm Table 2: Sperm Count: stock solution, 1:1 dilution, plastic container and Sea Uchin. Stock solution 2,310,000 1:1 dilution 4,620,000 Plastic container 13,860,000 Sea Urchin 1,663,200,00 Discussion The size and amount of eggs and sperm produced are vastly different. The amount of sperm produced is a reproduction mechanism to…

iodine solution. I will be testing the antiseptics at the concentration they already have because these antiseptics are bought at that concentration and their practical use usually does not involve additional dilutions. The E.coli bacteria will be diluted using serial dilutions; serial dilutions are necessary in the process of recording how much bacteria there is in a given sample and it prevents bacterial cells from clumping. Some of the sources…

occurred during the incubation and storage of the nutrient agar plates. In addition to this, samples of Streptomyces were originally planned to be taken from the 10-6 dilution plate. In transfer from the incubation plate to the inoculation plate, two (2) sample Streptomyces colonies fell onto the bench. Colonies from the 10-4 dilution were then…