Dilution

Table 1 provides the statistics for the first part of lab 11. In this section we used a 1:5 dilution of AdhP from our elution two. Using a spectrophotometer set to 280nm, the concentration of this dilution was found to be 0.08725uM and would be mixed with the various amounts of EtOH and NAD+. When mixed we were then allowed to observe the kinetics using spectroscopy set at 340nm. The data can be seen in Table 1 and Table 2. We were the able to plot this data using Excel thus creating Figures 1…

calculate the cell count and growth of bacteria, while using serial dilution. The 3 techniques were used to calculate the % viability of Serratia marcesens in a solution. Introduction Enumeration of microorganism is used to determine the amount of bacteria present in a sample. Microbiologists around the world are using enumeration to calculate the amount of bacteria present in drinking water and other potable drinks. Serial dilution is used to lower the amount of bacteria present in a…

MRSA+VRSA and four MRSA+VSSA) were analyzed through determination of their minimum inhibitory concentrations (MIC) values, minimum bactericidal concentrations (MBC) values, and MBC/MIC ratio by broth micro-dilution method using 96-well micro-titer plates. To each 50 μL of the antibacterial agent dilution, 50 μL of adjusted bacterial concentration inoculum (5 × 105 CFU/mL) were added in the 96-well micro-titer plates, the growth control wells contained MH broth medium with tested bacterial…

to work with salt water and gummy bears. Our experiment tested the weight of the gummy bears per salt dilutions. Our tests included 1g 2.5g 5g, 1.5g, and 10g of salt diluted with water. We knew that the higher the concentration of salt diluted into the water the heavier the diffused gummy bear would be, therefore we measure weight as our numerical data. My hypothesis was that the 10g dilution of salt would have the gummy bear with the largest mass. This was because diffusion is the process…

because it has a contractile tail” (Yap, M., & Rossman, M., 2014). Each tube was labeled with dilution and contained a red or yellow cap. First 100μl of the T4 virus was added to the first tube with 9.9 mL of phage buffer. Then 1mL of the dilution in tube 1 was added to tube 2 with 9mL of phage buffer, 1mL was taken from tube 2 to tube 3, and 1mL was taken from tube 3 to tube 4. Next 1mL of the dilution in tube 2 was mixed with 500μl of E. coli in a tube labeled 10-4, 1mL from tube 3 was added…

A set of serial dilutions is made from water sample collected, a sample of each is placed into a liquefied agar medium, and the medium poured into a petri dish. Dilutions are designed in order to make the calculation of the number of cells/ml in the original sample easier. The most common dilutions are 1/10, 1/100, and 1/1000. After incubation, agar plates with 30-300 colonies are counted. Greater than 300…

Materials and Methods Materials used in experiment 1.1A: Nutrient agar plate Materials used in experiment 1.1B: Nutrient agar plate, Sterile cotton swab In experiment 1.1B a nutrient agar plate was opened and left in an arbitrary spot in the laboratory and left untouched for three hours, at the end of the lab it was collected and incubated at . In part B a swab/swab rinse method was preformed in order to inoculate the nutrient agar plate. A sterile cotton swab was taken out of its packaging, an…

antimicrobial concentrations (Jenkins and Schuetz,2012). Both micro- and macro- dilution methods, present the lowest concentration where it is completely inhibited and is recorded as the minimal inhibitory concentration (MIC). MIC is the minimum concentration of an antibiotic that will inhibit the visible growth of a microorganism (McKenzie,2009). The procedure for macrodilution involves preparing two-fold dilutions of antibiotics in a growth medium and dispensed into the test tubes. A…

two, it would imply that there are other matrices affect the Roundup. When looking at figure 2 and figure 4, the calibration curves are similar. This can be seen when looking at the dilution 0.2% where both graphs are above an absorbance of 1. Also, the relative deviations are small. With both the serial and stock dilution, the pH was the same. The purpose of determining the pH is for a future test. The pH was relatively the same as the distilled water. When testing the roundup at 100%, the pH…

Meaning where each dilution is going to take place and where to adding each substance into. In this case: Row A: was skipped completely, Row B: Columns 1-6 were for the untreated control cells (cells only in normal media), Row B: Columns 10-12 were for treated cells with plumbagin, Rows C and D: were for cells treated with doubling dilution of our reagent/chemical, and lastly Rows E and F: were for cells treated with doubling dilutions of the vehicle. After knowing where each…