Dilution

Lab number 11 is also titled “Final Plaque-Purification”. The objective for this lab is the same as lab number 9. The materials needed in this lab are a sterilized Agar plate, sterilized, 1 P20 Micropipette. P20 micropipette tips, 4.5 milliliters of Top Ager, 0.5 milliliters of M. smegmatis, 2 serological pipets, and 5.0 milliliters of Phage buffer. The protocol is to create agreed on the bottom of the Agar plate to look like a checkerboard. Flip it back over with a cover face in the ceiling.…

20%, 20%, to 0%. This supports the hypothesis because the greater dilutions had a higher mortality rate while the smaller dilutions had lower mortality rates. However, the upstream data was also convincing. The greatest dilution, 1:4, had a mortality rate of 80%, which is greater than on site. This could be considered an outlier because it seems random compared to the rest of the upstream dilution rates. However, the smaller dilutions, other than 40%, had mortality rates of 0%. This seems…

Listeria monocytogenes is a Gram-positive rod-shaped bacterium. It is the agent of listeriosis, a serious infection caused by eating food contaminated with the bacteria (Todar, 2008). The disease primarily affects older adults, pregnant women, new born, and adults with weakened immune systems. Babies can be born with listeriosis if their mothers eat contaminated food during pregnancy. However, healthy people may consume contaminated foods without becoming ill. Listeria monocytogenes is commonly…

The Carbohydrate Fermentation Test measured the ability of the bacteria to ferment glucose and mannitol carbohydrate and produce gas. Majority of microorganisms obtain energy from either aerobic or anaerobic respiration, or through fermentation. The different types of fermentation pathways used by bacteria can aid in differentiating them among groups or species of bacteria because each pathway depends on the complement of enzymes available for the cell (Department of Biological Sciences, 2015).…

1125(a); trademark dilution under the Trademark Dilution Act, 15 U.S.C. § 1125(c); state trademark infringement under Va.Code § 59.1–92.12; and common law unfair competition”. Issue: The issue of this case is that Beehive Wholesale sells a brand of watches called “SWAP” and put in an application to register its mark but Swatch feels as if the application should be reversed because it would be a violation of “federal, state, and common law trademark infringement, trademark dilution, and unfair…

Illustration of sexduction in E. coli using streptomycin resistant F-lac- recipients and F′lac+ donors Abstract The process in which DNA from a donor cell is transferred through physical contact into a recipient cell is known as conjugation. In the experiment, sexduction, a cross between F′ and F- plasmids, was demonstrated by mixing the donor and recipient, diluting them and using selective plating methods. The plasmids from the donor, recipient and transconjugant were isolated and analyzed…

volumetric flasks, and distilled water, the three dilutions were prepared. First, a 5mL volumetric pipet was used to put 5mL of the red dye stock solution into the first 10mL volumetric flask. Next, 5mL of distilled water was added to the first 10mL volumetric flask to create was dilution #1. For the second 10mL volumetric flask, 5mL of dilution #1 and 5mL of distilled water were added to created dilution #2. Lastly, to create dilution #3, 5mL of dilution #2 and 5mL of distilled water were added…

the bacteria were diluted via serial dilution in order to more accurately count and quantify the bacteria. To ensure that the three different strains of bacteria are not cross contaminated, label everything either Farm A, Farm B, or Farm C depending on what is being tested. There are six agar plates for each farm, three of the plates are tetracycline plates (which are labeled) and three are unlabeled. For the three labeled plates, mark each with their dilution which will be: 10-2 ,10-4, or…

Introduction: The ability of substances within a solution to absorb light is a wonderful phenomenon as it aids in processes such as determining the concentration of proteins. The way that a solution absorbs light is due to the photoelectric effect. The way the photoelectric effect works is that when light comes in contact with the sample, the photons of light will react with its electrons. As the light travels through the solution, "a photon of light may interact with an electron ... [where]…

all recorded data would have the same units for comparison and is needed due to the fact that the basic unit for concentration is mg/ml. Table 3: Calculated Protein Concentrations Sample Initial Absorbance (OD) Dilution Absorbance (1:50) with DI water Calculated Concentration with dilution in consideration(mg/ml) Concentration on Label (mg/ml) Protein Shake 1.912 0.164 13.667 21.1 Whole Milk 2.391 0.431 35.914 33.8 Muscle Milk 2.176 0.700 58.333…