Dilution

The purpose of the research was to potentially find a new bacteria that is capable of producing an antibiotic against one or more of the six ESKAPE pathogens (a group of pathogens that are resistant to most if not all current antibiotics). All research protocol was based on the Yale Small World Initiative and the third edition of Microbiology Laboratory Theory & Application. One bacteria from a sample of soil collected (colony #18) was tested for its ability to produce antibiotics against two of…

many viruses are in a culture. During this lab this Plaque Counting Assay was used to determine how many viruses were in the solution. The lab was to conduct a serial dilution to determine the titer of the bacteriophage…

from -0.0005 to -9E-05, implying a decrease in metabolic rates. This discrepancy may have been caused by different factors throughout the lab. The biggest concern with the validity of this lab would involve the dilution of the nicotine infused water in the second trial. Although the dilution may have skewed the data, it was a necessary step to ensure the safety of the…

There are factors that can affect the enzymatic activity. Enzymes are known to speed up the biological reactions. It is specific to its substrate which in this experiment is known to be catechol. The catechol oxidase is used in all parts to express the concentration in the enzyme reacting into ortho-quinone (Schultz, 2006). The catechol oxidase if from the potato that was used in this experiment. The enzyme was extracted as the full strength after centrifugation and used the homogenization as…

Staff Analysis Statement of the Problem In July 2011, Pacific Grove Spice Company (Pacific Grove) is experiencing a major problem regarding the large amount of debt it has accumulated in the following years. Debra Peterson, CEO of Pacific Grove, is envisioning on solutions to "reduce interest-bearing debt to less than 55% of total assets and the equity multiplier to be less than 2.7 times by June 30, 2012" (Pacific Grove Spice Company Case, p. 3). Currently, Pacific Grove has a total debt of…

Infectious Coryza Limited by the knowledge of microbes, infectious coryza was not regarded as a distinct avian disease until 1920s. In 1932, the causative agent was isolated and named as Haemophilus gallinarum afterwards. Based on the identification of Page in 1960s, H. paragallinarum was isolated and regarded as the true causative bacteria. However, recent researches have confirmed both these two species belong to Avibacterium genus and are both reason for the fowl coryza, while most researches…

Abstract For this experiment the amount of zinc in vitamin water had to be determined. To do this, three methods were applied. The first was by a calibration plot method, where zinc standards were prepared and from the absorbance of those standards a calibration curve was plotted, which in turn was used to determine the amount of zinc in vitamin water. The second method was standard addition. For this method, an un-spiked and spiked solution were prepared. The un-spiked solution only contained…

This study is about isolating phage. The first step was to do an enrichment isolation then do a plaque assay and spot test. After that the next protocol was do a plaque streak, which was conducted numerous of times. Then serial dilution was conducted and RE Digests of Phage DNA as well. Last step was protocol 10.3, gel electrophoresis. The relevance of this study is important to field of biology because bacterial infections could be treated with bacteriophages. Bacteriophages can be a solution…

1. Introduction In 1980, approximately 125’000 Cubans from Mariel Harbor arrived in the United States between April and October, leading to an increase of 7% of Miami’s workforce. The unexpectedness nature of the event, making it similar to a natural experiment, permitted David Card, a Canadian labor economist, to set up an innovative pre/post test design using a difference in difference estimator in his paper “The impact of the Mariel boatlift on the Miami labor market”. The latter study would…

Adalaide Kline A11796368 TR 10:30 1. Dr. Bronner’s Castle Liquid Soap is an all-natural multipurpose soap. This product can be used for faces, hair, cleaning dishes, mopping the floor, and many more activities. Personally, I use this product for my hair. I came across it when I needed to find a new shampoo that did not weigh my hair down and make it feel clean for a longer period of time. 2. Product: This product is an all-natural cleanser that has no chemicals in it. Other shampoo products…