Digestive enzyme

pH and temperature amylase activity from fungal and mammal Introduction The first enzyme that was produced industrially is amylase from a fungal source in 1894, it was used to treat digestive disorder. Amylase is an enzyme that breaks down starch into sugar and starts the process of chemical digestion in human salvia. Starch breakdown of amylase has received a great deal of attention because of their technological significance and economic benefits, and is also used for the commercial…

about, and the digestive hormones we are going to talk about, the body makes even more hormones for other reasons. Maybe we’ll have the chance to talk about some of them at a later time, but for now, let’s talk about digestive hormones.…

a trend in digestive system lengths correlate to an animal’s diet (Strack and Linzey.) Carnivores like wolves have less complex and less variety of diet (Strack and Linzey.) Unlike humans, wolves have smaller pancreas, which mean less enzymes produced(Griffiths and Ruish.) Wolves need to eat digested food with enzymes or easily digested food to increase absorption. That is the reason why they sometime eat feces and digested contents of a dead animal. Wolf’s saliva do not contain enzymes to…

ABSTRACT To examine the enzyme kinetics of chymotrypsin during the catalysis of the synthetic substrate p-nitrophenyl acetate (NPA). Initially, observe the magnitude of the initial burst with regards to the enzyme concentration. Secondly, the analysis of the steady state deacylation rate with regards to the pH of the reaction mixture. Chymotrypsin catalysis is a process that proceeds with two steps, an initial fast step involving the covalent modification of the enzyme to the substrate and the…

Proteins are known as the building blocks of cells in all living organisms. Many hormones are made up of proteins and the digestive system, immune system and blood replies on proteins. Therefore, protein is an essential part of healthy diet, and integral to development of the body. The purpose of performing this lab is to analyzing the activity of TEM-1 B-lactamase and how it reacts with high temperatures, chaotropic salts, detergents and pH values. INTRODUCTION: Primary protein structure is…

An enzyme is a macromolecule that acts as a catalyst in which it speeds up reactions by lowering activation energy (Campbell 151,152). Activation energy is the energy that is required for a reaction to take place (Campbell 152). In the reactions there are substrates which are reactants that enzymes act on (Campbell 153). Enzymes bind to the substrates then creating an enzyme-substrate complex, and while the two are joined together the catalytic action of the enzyme changes the substrate to the…

experiment, dialysis tubing was used to simulate the semipermeable plasma membrane of a caterpillar’s intestine. Cooked starch was prepared and mixed with the enzyme amylase to simulate the mixing of the caterpillar’s saliva with a starch/leaf and then placed in the dialysis tubing to simulate the beginning of the digestive process. The enzyme amylase breaks down starch into glucose and that glucose then is able to permeate the membrane of the caterpillar’s intestine. Lugol’s solution is able to…

needed for the digestion of fats in food. Made by the liver bile flows into the larger hepatic ducts and goes into the gallbladder where it is stored and can be used by the body later • Your pancreas is one of the organs that secretes enzymes into the duodenum. The enzymes found in pancreatic juice break down all of the major nutrients, including carbohydrates, proteins and fats. However, the pancreas needs a little help from the liver and gallbladder when it comes to breaking down fats Liver:…

intestine, and then to the large intestine. 2. How long does the food remain in each segment of the digestive tract and what physiological processing takes place during this time? • Chemical and mechanical digestion begins in the oral cavity. Temperature, taste, texture are sensed, and the saliva helps the chemical digestion because it is secreted by the salivary glands. The breakdown of the enzymes in the saliva helps the food to be swallowed. The mechanical breakdown of the food is from the…

Aim The aim of this experiment is to discover how different number of pH levels react towards the enzyme catalase, found in beef liver, and will be discovered by the number of oxygen bubbles through the degradation of hydrogen peroxide. Hypothesis The higher the pH level, the more production of oxygen bubbles in the solution. Variables Could affect activity of catalase - Temperature of reactant vessel - Concentration of Hydrogen Peroxide/Catalase - pH level of…