Digestive enzyme

INTRODUCTION: What is DNA? DNA is a deoxyribonucleic acid, which is a self-replicating material that is present in just about all living organisms as the top chromosomes. DNA Isolation is the development of purification of DNA from a sampling applying a mixture of physical and chemical methods. Isolation is a routine process in molecular biology or in molecular analyses. Materials: • Electrophoresis chamber • Gel casting trays • Sample combs • Ethidium bromide • Loading buffer •…

The egg is an example of an animal cell that is placed in environments of different tonicity. The vinegar was used to dissolve the shell so that we can see the effects when we placed the egg either in a hypertonic or hypotonic solution. Since the egg membrane is semipermeable similar to the cell membrane, it only allows specific substances to pass in and out of the egg membrane down its concentration gradient. In this case, we are trying to measure to the diffusion of water that goes in and out…

In conclusion, the purpose of The Hot box device is to make it so it can use energy from from the sun to allow the Cocina del Sol Company to bake their specialty cookies while staying eco friendly. My product used aluminum foil and black paper to attract heat to my device and what’s inside of it. We heated up the box by putting it in contact with light. You can do this by letting it sit in the sun or using a lamp or anything that gives off light like one. The results from my experiments…

Abstract: An enzyme, a protein, is a catalyst, which increases the rate of the chemical reaction. The results of this experiment showed how different temperatures could affect the chemical reaction between the enzyme and the hydrogen peroxide. The temperatures tested in this lab were 30, 35,40,45 Celsius, which are close to the average body temperature which is 37 Celsius. Catalase is an enzyme which breaks down hydrogen peroxide into oxygen and water. Different temperatures were measured to see…

When conducting the different tests and streaking, aseptic techniques were followed. For every T-streaked that was done, the inoculating loop was first flame for 5 seconds and was allowed to cool for 30 seconds. Afterwards the tube containing the mixed unknown bacteria was then passed over the flame. The inoculating loop was then inserted into the broth and then carefully streak on quadrant one of the plate. Afterwards, the loop was sterilized by the flame and was allow to cool for thirty…

Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done…

What is a catalase? A catalase is an enzyme that destroys harmful substances inside your body, and is a form of protein. (Corsini, "What Is a Catalase?") What is an enzyme? Enzymes are substances that speed up chemical reactions. (Corsini, "What Is a Catalase?”) This experiment involved both of these two aspects. It was inferred before the experiment began that if the potato is placed in hydrogen peroxide at room temperature, then it will produce the most bubbling activity. The reason that the…

Initially, the protein extract solution was subject to ion exchange column chromatography using SP Sepharose Fast Flow (GE Healthcare, Sweden). The column (with 5 ml of stationary phase) was equilibrated with five column volumes of sodium acetate/acetic acid 10mM pH5 and 10 ml of protein extract containing bacteriocin were injected into this. After washing the column with sodium acetate/acetic acid 10 mM pH5 (Buffer A), elution was performed using a linear gradient from sodium acetate/acetic…

This test is performed on organisms to see if it has the enzymes to reduce the Nitrate to Nitrite. To run the test, it will require using two colorless chemicals (so make sure you are paying attention) that will either change the color of the organism by the interaction of the new chemicals with the enzymes found in the unknown organism. So, if the organism has the capability to reduce the nitrate to nitrite the solution in the test tube will turn red almost immediately and the organism is…

from the cells, subsequently followed by excretion into bile or urine1. The metabolic enzymes in charge of this process are traditionally organized into two separate categories; phase I (oxidation, reduction, or hydrolysis) and phase II (conjugation) enzymes1,2. During phase I, polar groups are added to the molecules through oxidation, reduction, and hydrolysis, mediated generally through cytochrome p450 enzymes. Phase II…