Digestive enzyme

Results and Discussion 3.1 Composition of wheat starch granules The composition of starches and their separated A- and B- granules of two varieties are shown in Table 1. Amylose content of starches lies between 16.96 -24.69. The B-granules of the both varieties showed the lower value of amylose content while A- granules attributed to the high amylopectin to amylose ratio. Swelling power results the ability of starch to hydrate in specific cooking and heating process (90 °C/ 30 min.). The…

Our goal of the dialysis experiment was firstly, to answer the question of why the dialysis cells appeared smaller after adding sugar water and secondly, to prove our hypothesis that water molecules move out of the cell because the concentration of water is greater than inside the cell than it is outside the cell. This experiment was conducted with one control group and one experimental group. The independent variable was the concentration of water, (water and glucose solution). The dependent…

3.4.2.1 Test for milk:- 1. Determination of SNF in milk AIM: - to determine the SNF of milk Apparatus: - lactometer jar, lactometer and thermometer Principle: - It is calculated by using lactometer method. Procedure:-  Warm the milk sample to 40 to 45oCand maintain at this temperature till 5 min.  Mix the contents by rotating and inverting the bottle, taking care to avoid the formation of air bubbles.  Cool the sample to 15.5oC  Invert the sample bottle 2 to 3 times; pour enough milk into…

Discussion Nine of the Burpee Pea Seeds were tested with tap water that had a pH 8 and nine were tested with a lemon juice and water solution that had a pH of 4. of Between days one through four the acid rain and tap water seeds were alike. The seeds were round and tan. On day two the seed coat became squishy. On day three test one seeds two and three of the acid rain and water began to sprout roots, and all of the seeds in test two and three began to sprout. Seeds one, two, and three in the…

Background: Isopods, or what are often called pill bugs, are crustaceans. Isopods live in damp environments. They can be found under rocks, logs and in soil. In this lab, 10 isopods were given a choice between salt water or regular water chambers to test their preference. The results were recorded every 30 seconds over a period of 10 minutes. Problem: It is known that isopods have a preference for moisture.This lab will specifically test whether or not an additive will affect a pill bug's…

The second part involves finding the best speed for optimization. Eight different tests will be performed. Four in the first part and four in the second part. Each test will involve about 800 mL of the sample. In the first part, four tests were created of alum concentrations of 0, 5, 20, and 40 mg/L. All were subjected to the stirring apparatus at the same speed of 30 rpm for 15 minutes to allow for flocculation. Initial turbidities were taken before starting the mixing. After being mixed, they…

by denaturing the non-recombinant E.Coli proteins. The Second step was much more beneficial to the total purification but the combination of the two steps achieved an even better purification of 4.6 fold. From this assay we can see that the correct enzyme has been successfully purified. Considering the SDS-PAGE results we can confidently say that the purification steps were effective at removing a large proportion of E.Coli proteins. However, not all E.Coli proteins have been removed as shown by…

In our hypothesis we predicted that if borax is added to a laminated rubber-plastic composite it will strengthen it and the end product will have a higher puncture strength. From the data that we got by testing our plastic, it is maybe supported, but I cannot confidently say it is true judged by how weak our all data inclination is. The overall higher puncture strength is when we added 12 mL of borax to our laminated plastic. It may have been because borax is sticky substance and can cause the…

Rabia Iqbal Biol 395-1005 lab 01/31/2015 Broadford assay Absorbance Figure 1. The concentration and absorbance of Bradford assay. Four samples containing Bovine serum albumin, diluent, and Bradford. At 0.25 mg/ml concentration the absorbance was 0.109 nm and the relation between absorbance and the concentration held constant throughout except at the 0.50 mg/ml when the concentration absorbance was 0.186 nm. Figure showed that as the concentration is increased the absorbance elevates also. All…

First Experiment- One lab partner should prepare a test tube that contains 10 mL of the lactose solution. Make sure to label the tube as being Tube One. Another lab partner should prepare a test tube with 10 mL of the lactose solution and 1 mL of the lactase solution. Take caution in measuring the solutions. Label the tube with lactase and lactose as being Tube Two and cut off an appropriate amount of paraffin wax paper to cover the opening of the tube. Press the paper tightly to the tub and…