Digestive enzyme

1.)During the lab and based on recorded observations it can be deduced that the enzyme catalase found in potatoes only reacted to hydrogen peroxide. This is do to the fact that before 10 ml of hydrogen peroxide was added to each test tube residing 3 potato samples and the designated liquid, no reaction was observed. Thus demonstrating the catalase's inability to react to vinegar, soda, water, detergent, and Na OH. Furthermore, shortly after the hydrogen peroxide was added to the liquids of…

each time mark, which led to varied enzymatic activity and inconclusive or atypical results. Slight water residue was observed in the test tubes prior to use in the experiment, and may have contributed to the overall lack of enzyme activity (i.e. water may have diluted the enzymes). The iodine solution added to the spot plates just before the amylase-starch solution was added may have also contributed to the inconclusive results as it may have oxidized too quickly and the bond it should have…

substrate concentration (%N × 6.25), (ii) enzyme-substrate ratio (E/S) in % or in activity units per kg N × 6.25, (iii) pH, (iv) temperature, and (v) time (Adler-Nissen, 1984). Generally, there is an optimum combination of both pH and temerature, where the enzyme shows the highest activity. Physicochemical conditions of the hydrolysis reaction such as temperature, pH and enzyme/substrate ratio must be adjusted to optimize the activity of the proteolytic enzyme (Kim & Wijesekara, 2010; Santos,…

Introduction Enzymes are an important biological catalyst that is absolutely crucial for life to exist. The ultimate purpose of this experiment was to examine enzymes and how they function in cells, explore the result that concentrations and environmental factors have over these functions, and lastly to read and interpret graphs related to enzyme function. This type of study had various parts, therefore there are various hypotheses. The first being, if it is potato extract, then it will contain…

When a blank containing boiled (denatured) enzyme instead of an active enzyme could be used but it would not be the better blank to use in this experiment. The better blank solution would have been the one with active enzyme because it would cause the spectrophotometer to measure the absorbance of every component in the blank except the substrate, creating a 100% transmittance. This allow the spectrophotometer to read everything that was not in the blank when the actual sample is inserted in…

when breaking down tofu Introduction: This practical looking for the change that temperature has on the enzyme activity of pepsin on tofu. Enzymes act as catalysts and help quicken the process of breaking down foods that are called substrates, in digestive systems(Rader, A., 2016). In this experiment the enzyme is pepsin and it breaks down proteins into peptides, it is a common in the digestive system and helps breakdown the proteins that are in foods(Unkown, 2016), the substrate is going to be…

that the bromelain is started stable at 25% of enzyme concentration to 100% of enzyme concentration, pH 4 to 11, and the temperature range from 4oC to 37oC. For the concentration, it could happen because the presence of bromelain could break down the gelatin which makes the not solidified formation of the solution. In the temperature test, the lowest temperature makes the bromelain become optimum temperature which is regarded favorable for the digestive process, and on the pH level test was…

Making it obvious to observe the unexpected results as the temperatures 2, 20 and 27 remained blue then 37 degrees changed to green and 60 degrees changed to orange. This experiment was carried out to investigate how temperature has an affect on enzyme activity. This was done by preparing beakers with amylase, starch and benedicts solution at multiple temperatures and observing the colour changes present. The results collected from this experiment were…

important to enzymes? If homeostasis is not maintained, how can enzyme function be affected? Enzymes and many other organisms rely on homeostasis to complete their functions. Maintaining homeostasis is important because organisms adapt to fit their current environment, and if that environment does not stay constant the organisms might not be able to function due to their specialization. If the environment is in a state of disarray, i.e., homeostasis is not maintained, then the enzymes could be…

Enzymes are organic catalysts that speed up reactions that occur by lowering the activation energy of the reactants. By lowering the activation energy, the chemical bonds are weakened, thus the reactions are much faster than without the catalyst. Enzymes are composed of proteins which shape dictates the function of the enzyme. On the enzyme it contains a part called the active site where the substrate binds and the chemical reaction undergoes. A substrate is the chemical involved in the…