Denaturation

was unable to hydrolyze the starch present in the solution. The enzyme’s denaturation led to the loss of its…

The Draize test, devised in 1944 is a widely used test that predicts human ocular irritancy by using laboratory animals, primarily albino rabbits. A substance is applied directly into the albino rabbit’s eye or to the surrounding skin to identify its likely effect on the corresponding human tissues based on direct visualization (Mitjans, Infante & Vinardell, 2008). Due to advances in ocular technology, the precision, validity, need and relevance of the Draize test is being challenged…

4. Multiplex Polymerase Chain Reaction Multiplex PCR is molecular biology technique for amplification of multiple targets in a single PCR reaction. Indeed, multiplex PCR is a modification of conventional or realtime PCR in which two or more different PCR products are amplified within a single reaction (Henegariu, 1997). In a multiplexing assay, more than one target sequence can be amplified using multiple primer pairs in a reaction mixture. As an extension to practical use of PCR, this technique…

My first research experience started when I participated in bone scaffold designing project at the Cooper Union in Spring 2012, under the supervision of professor David Wootton at Cooper Union Department of Mechanical Engineering. The purpose of this study was to design and manufacture bone scaffold for orthopedic trauma, especially for segmented bone fracture. I worked with a senior mechanical engineering student who had been conducting the research as his senior capstone project. The first…

Did you know that Jell-O has been a part of American culture for over a century? Jell-O, a dessert made from prepared powder, is something that we, humans, eat everyday. To keep a solidify figure, the Jell-O requires gelatin as a part of its ingredients. For my experiment, I will be determining whether certain enzymes in fruits prevent gelatin from the process of solidification, and if there’s a way to still include these fruits without ruining the dessert. The research question of the project…

1.5. Enzyme-Polysaccharide Interactions Proteins and enzymes have become an integral part of many industrial processes as well as being applied both in cosmetics and in therapeutics. Due to advances in biotechnology and genetic engineering, mass production of protein and enzymes has become feasible. Use of enzymes is environmentally friendly as they catalyse reactions under mild conditions against polluting inorganic catalysts which works in the extremes of pH and temperature. However, this can…

start a biochemical reaction. According to Dr. Meyertholen (2015), enzymes are structured to work with certain substrates, meaning that the structure determines the function of the enzyme. If the enzymes shape is altered, they become denatured. Denaturation of an enzyme can occur with changes in factors such as temperature, pH, or salinity. The enzyme being used in this experiment is catalase. Catalase is an antioxidant enzyme that helps to…

Sofya Tkachman AP Biology, A2 Dr. Lau 15 September, 2015 Enzyme Catalysis I. Purpose Measure the activity of catalase in potato extract using several different methods. II. Procedure a) Cut 20 small circles from a large filter paper disk using a hole puncher. Clean and dry several medicine cups and pour hydrogen peroxide into one. Put the catalase enzyme, previously prepared by blending a potato, into another medicine cup. Make a 1/8 dilution of the catalase by adding chilled distilled water.…

reactions to proceed to form products. The rate of enzyme activity can be impacted by environmental conditions such as material concentration, temperature, and pH. Alterations of these conditions outside normal ranges for an enzyme results in denaturation or shape changes to active sites of enzymes. This results is decreased or cessation of function of the enzyme.(Reece, et al 2014) To investigate this, yeast catalase were incubated at varying temperatures to determine when decreased enzyme…

This hospital-based case-control study was conducted on 110 consecutively newly diagnosed LP patients and 120 apparently healthy, age and sex-matched, control subjects. The control group consisted of participants referred for non-inflammatory dermatological conditions such as chronic hair loss or coming with their relatives. They had no oral mucosal, systemic or autoimmune diseases, and had no history of LP. All subjects were recruited from the outpatient clinic of the Department of Dermatology,…