Cytotoxicity

NK-cells (natural killers) represent a heterogeneous lymphocytes population of innate immune system. They have a natural cytolytic activity, are capable of producing cytokines and chemokines and are involved in the antiviral and antitumor body control. In the quiescent state, an average diameter of NK-cell is about 8.7 microns, while in the moment of its activation it may increase its size up to 10-12 microns (Ferlazzo et al., 2004). This feature was the reason for the initial determination of…

bone-marrow cells. In contrary, CP extensively reduced the cell viability to 64.07±2.12% in comparison with 96.4±0.81% for non-treated cells. The serial administration of variable concentrations of FFCC concurrently with CP triggered reduction of cytotoxicity induced by CP alone. The percentages of cell viability increased significantly from 72.58±1.5% to 83.16±1.73% when animals simultaneously treated with CP and FFCC at 50 or 200 mg/kg b.wt respectively in comparison with CP alone (Figure…

Bakr et al Clin J Pain  Volume 00, Number 00, ’’ 2016 6 | www.clinicalpain.com Copyright r 2016 Wolters Kluwer Health, Inc. All rights reserved. Copyright r 2016 Wolters Kluwer Health, Inc. Unauthorized reproduction of this article is prohibited. was a significant decrease in CD4, CD8, and CD56 in group M, compared with groups T and K and in CD3, CD8, and CD56 in group T compared with group K at 90 minutes postoperatively. After 24 hours, there was a decrease in CD4, and CD8 in group M in…

promoting the polymerization of microtubules and prevents depolymerization to have a cytotoxic affect on cells (DeAngelis LM, et al., 1995). Omeprazole is a proton pump inhibitor used clinically for gastritis that inhibits V-ATPase and increases the cytotoxicity of chemotherapies such as paclitaxel (Bae DS, et al., 2015). The metastasis of cancerous cells is known to be the leading cause of mortality in cancer patients. Cancer cell progression and metastasis occurs when the cancerous cells are…

Hashimoto’s thyroiditis Case Study “Pathophysiology refers to the study of abnormalities in physiologic functioning of living beings” (Copstead and Banasik, 2013, p.1). Pathophysiology attempts to discover any responses and reactions of living being’s organs, tissues, cells and body fluids, in response changes experienced in its internal or external environment. Pathophysiology becomes extremely important in the medical setting as it allows us to predict expected outcomes of diseases…

Background: Large bone defects and infections still represent a major challenge to orthopedic surgeons. Bone infection is a very serious condition resulting in patient suffering, financial burden, and sometimes fatalities. The main cause for bone infection is usually trauma, diabetes and biomaterial-related implant associated infections. Infection is a significant cause for implant failure, impaired functionality, and reduced lifetime of medical devices, resulting in high distress for the…

3.1 The effect of H. sabdariffa calyx extract on the antioxidant property. Due to different parts of H. sabdariffa and extract in different ways, have different antioxidant activity. In some study, H. sabdariffa extracts from different parts were detected with some antioxidant capacity assays and determined that H. sabdariffa calyx has the high content of phenolic compounds associating to antioxidant, and have the significant effect on β-carotene bleaching. In freeze-drying, extract in cold…

4.2.3.5. Antioxidant assays in gastric tissue homogenate 4.2.3.5.1. Catalase activity Catalase activity in the gastric tissue was measured according to the procedures of Sinha (1972). One ml of homogenate solution was taken and mixed with 5 ml of phosphate buffer of pH 7.4. To this 4 ml of 0.2 M H2O2 was added and time noted. Exactly after 180 seconds of adding H2O2, a set of 1ml of reaction mixture from the above was taken and 2 ml dichromate acetic acid was added. It was then kept in a boiling…

Three differ assays were used and tested in this experiment: Antibacterial, anti-biofouling, and cytotoxicity assay (anticancer). Each assay used different microorganisms that were grown and tested in the lab, and were then introduced to various levels of synthesized AgNPs-- growing D. radiodurans strain R1 ATCCBAA-816, without the presence of oxygen in…

Other strategies include employment of cell cycle arresting drugs which inhibit PIk1 eg..,volasertib. Monoclonal antibodies can also be used in treating AML like SGN33A which is a DNA cross linking toxin and AMG330 which mediates cell mediated cytotoxicity against the leukoblast cells. Other approaches are employing iso-citrate synthase inhibitors to mediate cell death by atrophy. Despite all these approaches clonality is very strong in cancer cells and one of the most effective way is to…