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    Page 7 of 9 - About 85 Essays
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    GFP Tagging

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    In molecular biology, individuals use different techniques to obtain data for their research. These methods include His and GFP tagging which play an important role in experimental studies. His tagging is widely used for purifying and detecting large amounts of targeted proteins (Kaplow et al., 2009). GFP tagging can aid in the analysis of protein geography, movement and chemistry in a living cell (Lippincott-Schwartz and Patterson, 2003). A His-tag is a sequence of approximately six histidine…

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    diagnosis of brain diseases and support development of new brain therapeutics. References 1 Nakai J, Ohkura M, Imoto K. A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat Biotechnol 2001; 19: 137–41. 2 Nagai T, Sawano A, Park ES, Miyawaki A. Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc Natl Acad Sci U S A 2001; 98: 3197–3202. 3 Akerboom J, Rivera JDV, Rodr??guez Guilbe MM, Malav?? ECA, Hernandez HH, Tian L et al. Crystal…

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    Monday, the start of yet another school day. Another day where he 's absent. Another day not learning anything. Another day where he won 't see and talk to his friends again. The opened window brought in cool air, swirling around the average hospital room. Unfortunately, this made the eleven-year-old boy shiver nonstop and his teeth clattering like a skeleton would. His front bangs of his silver-dyed hair brushed upwards, for the air sometimes liked to mess around with it, showing the child 's…

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    Sound Design In Theatre

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    Since theatre first became popular in the times of the Greek, the use of sound to enhance theatrical experience has been prominent. However, many leaps have been made since then to improve a sound designer’s abilities to create different and intricate sounds in their design processes. The evolution of sound playback systems is a key element in the development of sound design in theatre. Through exploration of the history of sound playback, the current available playback systems on the market and…

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    Diagnosis of Neurofibromatosis type 1 is based mainly on clinical findings which meet the diagnostic criteria for Neurofibromatosis 1 which was developed by the National Institutes of Health. Since Neurofibromatosis 1 is caused by heterozygous pathogenic modifications in NF1, molecular testing is rarely needed for diagnosis, and is only useful for certain individuals. The diagnostic criteria for Neurofibromatosis 1 is: 1 they must have six or more café-au-lait macules which are, for prepubescent…

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    Gene Therapy Disadvantages

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    Gene Therapy: The concept of gene therapy emerged in the 1970s. The basic premise of gene therapy is using DNA enclosed in a vector as a therapeutic treatment for diseases. In the decades after it was conceptualized, many clinical trials of gene therapy were developed but stalled in phase I or phase II. During the 90s, only 1% of gene therapy trials made it to phase III and none of them went past phase III. The excitement for gene therapy was reduced when a participant of a clinical trial died…

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    Aspects of Humanity Revealed through Symbolism in Never Let Me Go Kazuo Ishiguro’s Never Let Me Go has been widely acclaimed for its insights into the depths of human life. On the surface, the main characters, Kathy H., Tommy, and Ruth live completely normal lives, but their inevitable futures as organ donors separates them from “normal” people. Throughout their childhood and adolescent lives, the characters encounter various objects that become sentimental to them. Kathy greatly values her…

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    practices. Transnationally, there are conflicting environmental recommendations and laws that can make it easy to do the least possible when it comes to being eco-friendly. Companies should make every attempt to follow the guidance of the Global Compact and use innovative technologies to prevent environmental damages. For example, many involved in the diamond industry are pursuing the expansion of synthetic or lab grown diamonds, which would eradicate the need for mining…

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    etc. It needs much of the organizational efforts to qualify the CSR results in human rights, protection of environment, relations with their co-workers and the society. At international level, GRI (Global Reporting Initiative) and UNGC (UN Global Compact) are taking initiatives for the CSR reporting and to create more transparency (GRI,…

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    Hst Observation

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    images contents. As the final portion of the lab, the researcher will review a NASA mission, to enable the understanding of tool, content, and simulation of the actual study. As a researcher, I began my lab by researching the Australia Telescope Compact Array (ATCA). The ATCA is operated by the Commonwealth Scientific and Industrial Research Organization (CSIRO) at the Paul Wild Observatory located west of Narrabri, Australia. The radio telescope is built up of six dishes all with identical…

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