Column

Initially, the protein extract solution was subject to ion exchange column chromatography using SP Sepharose Fast Flow (GE Healthcare, Sweden). The column (with 5 ml of stationary phase) was equilibrated with five column volumes of sodium acetate/acetic acid 10mM pH5 and 10 ml of protein extract containing bacteriocin were injected into this. After washing the column with sodium acetate/acetic acid 10 mM pH5 (Buffer A), elution was performed using a linear gradient from sodium acetate/acetic…

Evolutionists use the geological column as a means of supporting their theory of evolution. The geological column is the theory of a sequence of rocks extending through time from the oldest at the bottom to the youngest mud, dirt and sand deposited on the surface. In many locations around the world, fossils have been discovered that run through many layers of rock. These fossils are known as polystrate fossils. This occurs when a fossil is deposited quickly and through a very large event, such…

As mentioned earlier, the vertebral column consists of five regions. These regions are cervical (neck), thoracic (upper and mid back), lumbar (lower back), sacral and coccyx. Some of the structures that would be affected by the incident are the intervertebral discs, due to the compression that was placed on the head. If the vertebral discs structure is severely compressed, the inner material, known as the nucleus pulposus, that is inside the discs could leak out and cause herniation. Herniation…

Columns (or posts) are large, cylindrical pillars of stone mainly created to support beams or arches. Ancient Egyptian architecture made use of stone columns whose surface was carved to reflect the organic form of bundled reeds; in later Egyptian architecture faceted cylinders were also common. Columns in the ancient Mesopotamian region were in turn inspired by their palm trees, as it was a very fertile land. The columns were created for structural support. Columns are frequently used to support…

For the column project I was trying discover and understand the balance between terrestrial and aquatic habitats, how they support each other by the transmission of nutrients and oxygen between the two habitats. The goals that were devised in class were to observe the various cycles such as the nitrogen cycle, the phosphorus cycle, etc. My hypothesis for this experiment was that the terrestrial habitat would support the aquatic habitat by transmitting nutrients and other abiotic necessities, I…

thrive on the rocky shore. Column barnacle The column barnacle is a smaller barnacle than the brown barnacle. Its fundamental niche ranges from high to low tidal zones, but it is more prone to desiccation than Chamaesipho brunnea so it prefers a damper climate (Luckens, 1975). While the brown barnacle only settles on rock, the column barnacle can also settle on mollusc shells, wood, iron and other barnacles (Luckens, 1975). This is an adaptation that allows the column barnacle an advantage in…

It was on an early morning during the fall, a Monday. We were all getting ready for a “long” trip. The four of us, each carrying a thick, heavy duffle bag. Cramming in our oversized bags, only two could fit in the trunk of our small white sedan. The sedan was about as small as a smart car. We decided to put the remaining bags at our feet. “Shotgun,” I yelled. In the back of my mind, I knew calling for the front passenger seat was a bad idea when I saw one of the duffle bag taking up the whole…

Trajan’s Column, a Narration of the Military Might of Rome Trajan was the emperor of Rome from 98 to 117 AD. Trajan’s rule lead Rome to the largest military expansion in their history, by the time of his death, Rome had grown to a large size than any other emperor had maintained before (Norris). Before his rise to emperor, he had to be nominated as consul, which required the person to be of important status or made in society, and have the backing and trust from the senate (Robertson). He was…

Introduction Spinal stenosis is a condition which involves the narrowing of the vertebral column, either in the spinal canal, intervertebral foramina, or the intervertebral spaces. The condition is degenerative and widespread, affecting approximately five in every one thousand people (Hsiang, 2015). This report aims to describe the anatomy of the vertebral column and spinal cord, relating changes in anatomy to the development and presentation of spinal stenosis. Within this discussion, the…

Kahlo, The Broken Column is an outstanding piece of artwork crafted out of oil on Masonite. Kahlo decided to use a color palette that was decently limited as mostly using warmer colors. These colors that Kahlo is frequently using are broken up by cooler tones mostly in the sky that is having the audience pull their view right to Kahlo’s figure.…