Column chromatography

a strong peak with the molecular weight distribution of ̴ 78 which was a sum of one Cas1 dimer and one Cas 2 dimer. Thus these observation support the calculated stoichiometry from ITC data. Also, retention time of the complex on a gel-filtration column was also consistent with the AUC…

Pectin yield was determined as weight of dry pectin against the weight of initial residues multiplied by 100%. Y=100(P/Bi) Where Y is the extracted pectin yield (%), P is the amount of extracted pectin in grams and Bi is the initial amount of sweet potato residues used to extract pectin. Equivalent weight and Degree of methoxylation The equivalent weight was determined according to the method by Barretina et al., (Barretina et al. 2012). 0.25g pectin was wetted with 5ml ethanol and topped…

Interpretation of the NOESY: (Nuclear Overhauser Effect) spectrum of 1-(2-methyl-1,2,3,4-tetrahydroquinolin-4-yl)azepan-2-one (3a) is as follows. Strong coupling interaction of protons of 2nd and 4th position in 1-(2-methyl-1,2,3,4-tetrahydroquinolin-4-yl)azepan-2-one (3a), was not so strongly observed in COSY because it involves long range coupling i.e. protons attached between 2nd and 4th carbon atom. Hence, we studied NOESY to know the spacial interaction between these two protons. Thus, the…

1. Amlodipine is a medication used to lower blood pressure and prevent chest pain. It belongs to a group of medications known as long acting dihydropyridine-type calcium channel blockers. Amlodipine widen the blood vessels and improves blood flow. Widening of these blood vessels lowers blood pressure. In angina, amlodipine increases blood flow to the heart muscle to relieve pain due to angina. (WHO, 2013). Amlodipine was discovered by a research team lead by Simon Campbell and created by the…

Kanamycin sulphate and rifampicin were purchased from Hangzhou Dayangchem Co., Ltd., Zhejiang, China. Acetonitrile and ethanol (High performance liquid chromatography, HPLC, grade) were purchased from Merck, Germany. Sodium dihydrogen phosphate, phenylisocyanate, triethylamine, orthophosphoric acid (analytical reagent grade) and silicone oil (viscosity 10 cSt) were purchased from Sigma–Aldrich, St. Louis, USA. Size 3 hard gelatin capsules were kindly donated by Capsugel Co., Ltd., Tokyo, Japan.…

Chitin is the second most abundant natural polysaccharide consisting of β(1→ 4)-linked N-acetyl-D-glucosamine (GlcNAc) units in a linear form. Chitin is insoluble in water and mainly exists in two crystalline (α- and β-) forms. The α-chitin consists of sheets of tightly packed alternating parallel and antiparallel chains (1) and is abundant in the exoskeleton of arthropods, insects, fungi, and yeast cell walls. The chains are arranged in parallel orientation in β-chitin (2), which occurs less…

Mesalazine and Rifaximin use for Inflammatory Bowel Disease. Mesalazine is used as in anti-inflammatory agent, Steroidal. Rifaximin is used in Gastrointestinal Agents, Anti-infective agent. The use of Rifaximin in combination with Mesalazine has been proved to provide beneficial effect in inflammatory bowel disease. The mechanism of Mesalazine and Rifaximin is quite different. Mesalamine and Rifaximin are two different types of drugs offering some symptomatic relief to the IBD patients.…

2. Materials Haffkine Biopharmaceutical Corporation Limited (HBPCL), Maharashtra, India, made available the venoms of Indian cobra (CV), Common krait (KV), Russell’s viper (RV), and Saw scaled viper (EV) and ASVA. The venoms were distributed in small aliquots, lyophilized, and stored at -20o C. Adult male Swiss albino mice (18 - 20 g) and New Zealand white male rabbits (2.0 - 3.0 kg) were maintained at HBPCL, Maharashtra, India and used for experimentation. Complete Freund 's adjuvant (CFA),…

1. Introduction L-Ornithine is an important chemical ingredient for its applications in food and pharmaceutical industries as a dietary supplement, as it is known to be efficacious in the treatment of liver diseases and wound healing [11]. It can also be used as a precursor for the synthesis of other amino acids, such as arginine and citrulline, and putrescine, an important diamine used as a nylon precursor [28]. L-Ornithine could be produced by fermentation using auxotrophic mutants, for…

Your eyes open as you hear the loud slam of the doors behind you. You try to look around, but find yourself unable to move. You see mysterious silhouettes standing over you with needles in their hands as you hear the squeak of wheels against the floor. A man comes near you and starts saying words you cannot seem to make out. Everything slowly fades to black, and you are out. You wake up hours later in a hospital bed, and the same mysterious man comes out from behind the curtains. After a long…