Column chromatography

4.3 High Performance Liquid Chromatography (HPLC) An HPLC analysis was carried out for the Fraction C2-7 for confirmation of the results obtained by TLC analysis. According to the HPLC chromatogram, there is only one peak was observed at the retention time of 4.510 minutes. Therefore, it can be concluded that there is only one compound presents in the Fraction C2-7. Basically, the first few small peaks in the chromatogram which is around 2-3 minutes are ‘noise’ from the injection that been made…

1. Abstract In this study, reverse micellar extraction of asparaginase model system was carried out using CTAB/Iso-octane/hexanol/butanol system to optimize the forward and back extraction efficiency. By using Taguchi’s orthogonal array (OA), maximum forward extraction efficiency of 55.0%, 61.0% and 54% was achieved with an aqueous phase pH of 11.0, 150mM CTAB/Iso-octane and 0.1 M NaCl, respectively. Similarly, Taguchi’s orthogonal array (OA) was applied to optimize the pH of stripping phase,…

dependant of their size. Size exclusion chromatography (SEC), is the controlled separation technique that is based on the size of the particles in relation to the pore size (Barth and Saunders 2012). The separation process veers to be quick for large molecules and slower for smaller molecules. The process would involve the large macromolecules to permit through the column bed, as the smaller molecules infiltrated the pores. Molecules passing through the column bed are divided between the…

Twenty Korean barley cultivars (Geongang, Gwangan, Nackyoung, Dahyang, Daejin, Saegang, Boanchal, Keunal, Saessal, Saechalssal, Sujung, and Boanchalssal) were selected in this research. All the cultivars were developed by the National Institute of Crop Science (NICS), Rural Development Administration (RDA), Korea and were grown during 2013 in the experimental fields of the NICS, Milyang, Gyeongnam. After harvesting, barley leaves were immediately washed with clean sterile water and then…

The past chapter proposed the continuous floating constraint and buckling column concept. In this chapter, a test setup is proposed to investigate its loading nature and validate the concept. A series of tests are performed, and the results are presented and discussed. The results of the tests conducted in this chapter will serve as measures in assessing the strengths and flaws of the initial concept, and point towards potential solutions. This group of tests varied two geometric parameters:…

Part A Gas Chromatography What is it? Gas chromatography is a variation of chromatography used in analytical chemistry for separating and analysing components that can be vaporised without decomposition ("Gas Chromatography", 2016). This technique involves using a gas as the mobile phase. There are two types of gas chromatography: • Gas-solid chromatography – separates substances on the basis of their different strengths of adsorption on the solid • Gas-liquid chromatography – separates…

perceptions of art by means of architecture and artisanal craft. While stylistically, architectural figures such as columns have not changed much from antiquity to modern times, the meaning and function of columns has developed a new meaning. Columns in antiquity provided support to a structure, while the styles of the columns told what type of structure it was. Today, there are columns that provide no functional support and are there purely for decoration. Works on pottery depicting a story in…

What is a relational database? It is a database that stores the information about both data, for example for a person detail that is link with their car details that they have bought it from the company. The data and relationships are showed in an entity which is in a flat, two dimensional tables. We would use a relational database in a company such as a car company so when they want to store their customers information and what car did they buy, they use a relational database which stores the…

Chromatography is a separation method used to help identify mixtures. One common form of chromatography is gas chromatography. This type of chromatography will usually use a gas as the mobile phase, and a liquid as the stationary phase (“Gas Chromatography”). A gas chromatograph is comprised of some vital components, which include: the carrier gas, flow controller, injector port, column, column oven, detector, and recorder (“Gas Chromatography”). The purpose of this lab was for an unknown…

are their columns. They inherited them from the Greeks and each style is generally dictated by the capital at the top of the column. The Romans used them to commemorate people, places, or even events by carving into the columns. They would decorate the columns after being built and put them into place, often taking years to finish. They had three major orders of columns the Doric order, Ionic Order, and the Corinthian Order. The Doric Order is the oldest and most simplistic of their columns.…