Column chromatography

The objective of the present study is to isolate piperine from the black pepper. Our attempt was to extract the piperine with petroleum ether from black pepper and isolation using the same solvent. It is very difficult to extract and isolate the piperine using a non-polar solvent like petroleum ether due to solubility. During our study, we observe that piperine have a very little solubility in the petroleum ether, at the same time the solubility of the resinous material in petroleum ether is…

Jain, 2013). The metal ions used in the filter are zinc, cobalt, nickel, or copper, due to histidines high binding affinity for these ions (Darain et al., 2004; Bornhorst and Falke, 2010). After the rest of the mixture has been washed out of the IMAC column and disposed of, a chemical wash buffer is used to displace the histidine tagged proteins from the matrix surface, allowing them to elute out by changing the binding affinity histidine has for the metal ions (Bornhorst and Falke, 2010). To…

Preparation of coating solutions Chitosan solution was prepared with 2 % (w/v) chitosan in 1% (v/v) acetic acid glacial. The solution was placed on a hot plate/magnetic stirrer for 6 h. The resultant chitosan-based coating solution was filtrated, through a Whatman filter paper No: 2, glycerol was added at 0.75 % (w/v), and stirred for more than 15 minutes. Two of the best concentrations, 60 and 90 mg mL−1 were prepared from freeze-dried IgY for S. putrefaciens and P. fluorescens respectively.…

measuring the electron transfer of inclusion complexes between the cucurbit[7]uril host. During my time doing research, I learned to synthesize macromolecules, to use instruments like the "pH meter", different methods for separation like column chromatography, and also to test the purity and concentration of a compound. I’ve discovered a world within chemistry that has helped me in the laboratory because in order to work there I had to master basic…

The column was Rtx-5 capillary columns (60 m ´ 0.32 mm, 0.25 μm film thickness). Helium (He) was the carrier gas at a flow rate 1.0 mL/min. The GC was interfaced with (Perkin Elmer Clarus 500) mass detector operating in the EI+ mode. The mass spectra were recorded over 40-500…

and methanol respectively. TLC analysis of the ethyl acetate fraction revealed the characteristic blue spots of polyketides with vanillin/sulfuric spraying reagent as well as the positive results with Dragendorff’s reagent. Using silica gel column chromatography run with dichloromethane and methanol, pulchranin A was detected in the first few fractions F5 to F10 with Rf value…

The column temperature was maintained at 36°C and 212 the detection source was ESI+ (electrospray ionisation). The solvents Eluent A2 (100 mL eluent A 213 concentrate [6-aminoquinolyl-N-hydroxysuccinimidyl carbamate + acetonitrile] and 900 mL 214 distilled water)…

Localization of Antidiabetic Potential of Cassia Tora Linn. Seeds Abstract Cassia tora Linn. is the wild medicinal plant and grows in most parts of India as weeds. In traditional literature, it is found that seeds of Cassia tora Linn. were used as folk medicine for the treatment of Diabetes in different parts of the world. In preliminary phytochemical investigations methanolic extract showed positive tests to sterols, carbohydrates, flavanoids, phenolic compounds, saponins and anthraquinine…

has a low ß-lactam binding affinity. Therefore, it can mediate cell wall synthesis even in the presence of ß-lactam drugs (5). mecA Homolog mecC gene Recently, mec C gene was found to confer ß-lactam resistance in SCCmecXI-carrying S. aureus. The mec C gene was found to share 70% similarity to mec A. Where as mec C encoded PBP2A homolog PBP2C, shares only 63% identity with PBP2A protein. In order to prove its role in Methicillin resistance, a knockout strain was generated.These stains have…

have a bioscience degree and proven relevant laboratory experience Experience with microbiological bench techniques or suitable transferable bench skills Experience of working within a GMP environment would be advantageous. Experience with column chromatography would be…