Column chromatography

Peak analysis was performed on Chromgate v 3.3.2 software. The diluted fraction was injected to appropriate concentration on Analytical C18 column (Coulter model, made in America). This column has the capacity to accept 165 µg. The column had the following characteristics (particle size: 5 µ, pore size: 300 Å, column size: 4/6 ꒼250mm). For conopeptide isolation, deionized water and acetonitrile, both containing 0/05%TFA with program (0 to 90% acetonitrile) for 90 minutes at a…

Experimental Method: Extracting LDH. The LDH used was extracted from bovine skeletal muscle tissue. In the cold room, a mass of 20 grams of muscle tissue was obtained. It was placed in a blender along with ice cold buffer, 50 mM sodium phosphate and 10 mM EDTA at pH 7.5. The mixture was then blended and homogenized in order to break open the cells; during this time the temperature was checked periodically to make sure it stays below 20 C. This was done until the solution was homogeneous. The…

Ammonium sulfate precipitation, ion exchange chromatography, and SDS-Page were the three different techniques used in this experiment. The standard molecular weight of rubisco is 66 kDa and 14 kDa. rubisco was expected in P2 with nothing added. Also in P2 medium salt the evident of rubisco. T The standard…

Aim: Discuss the advantages, disadvantages and limitations of mass spectrometry for the analysis of both small molecules and proteins. Background Mass spectrometers. They are a powerful tool used to evaluate analytes such as single atoms, molecules, whole proteins, or the simple peptide chains that make them up. Using the mass to charge ratio (m/z) of these analytes, we can differentiate one or many entities (such as one particular peptide fragment, or a panel of them) from everything else in…

Since suitable crystals for X-ray single crystal analysis was not obtained, to have reliable structural information, HR-TEM analysis of the product was carried out. The average size of the assembly calculated from HR-TEM measurement is found to be 2.8 nm which is in good agreement with the size of the previously reported Pd6L8 assembly where L is the non N-methylated (2.5nm, Fig. 2).9 As first step, to explore the catalytic activity of 1 in copper and phosphine free Sonogashira coupling reaction…

It is mainly used for identification of organic and inorganic substances found at the scenes of crimes. Some of the sub-sections include paper chromatography, electrophoresis, and DNA analysis. Paper chromatography in forensics is used to identify different kinds of drugs, food, and other substances. This is done by putting solutes in a mobile phase through a stationary phase by way of a solvent. This separates substances to…

Laboratory of Toxicology and HPLC of the Department of Clinical Laboratories, Pontificia Universidad Católica de Chile. Sphingolipid levels will be quantified by high performance liquid chromatography-triple quadrupole-tandem mass spectrometry (HPLC-MS/MS) on an Agilent 1200 HPLC system, equipped with an Agilent C18 column as described in protocol implemented and validated by our group2. Sphingolipid profiles will include the following molecular species: 1) sphinganine-1-phosphate and…

2. Experimental 2.1. Materials and reagents Analytical grade bentazon was purchased from Sigma-Aldrich. Acetonitrile, methanol, ethanol, chloroform and acetic acid were purchased from Merck and were analytical or HPLC grade. Methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EDMA) as the cross-linker and α,α′-Azoisobutyronitrile (AIBN) were purchased from Merck (Hohenbrunn, Germany). MAA was distilled under vacuum to remove the inhibitor prior to polymerization.…

Kate Byrne DT299/4 CHEM 4128 Applied Physical & Analytical Chemistry Assignment Liquid Chromatography High Resolution Mass Spectrometry Hyphenated Techniques The term “hyphenated technique” was introduced by Hirschfeld in 1980. It refers to the combination of a chromatographic separation technique and a spectroscopic detection technique to form a new, more efficent instrument. The technique which will be discussed in this case is LC-MS. The aim of combining both techniques is to purify and…

oligosaccharides is desirable yet the complete structural characterization of novel oligosaccharides remains challenging. The high polarity nature and highly branching, isomeric structures makes chromatographic separation with typical reversed-phase HPLC columns generally difficult. Moreover, oligosaccharide structures are usually in great diversity and the comprehensive structural characterization requires the determination of the degree of polymerization, the sequence of the constituent…