Column chromatography

Protein-protein interaction can be defined as the interactions between different protein molecules within the cellular organization leading to formation of large protein complex which is modulated by non-covalent interactions such as hydrophobic interaction, hydrogen bond, van der Waals interactions etc. Protein-protein interaction plays an important role in functioning of body’s significant mechanism such as replication, transcription, translation, signal transduction, cell cycle, etc. Thus,…

The objectives of this lab are relatively simple: using a separatory funnel to separate a pure substance from a mixture, determine the mass of a sample of caffeine using mass spectrometry, and as a way to separate compounds, use gas chromatography. Using gas chromatography and mass spectrometry you are more accurately able to determine the sample of caffeine. Amount of Caffeine in 100mL sample of Coffee. Introduction: Within this lab, our group was supposed to determine the amount of caffeine…

The experiment calls for the extraction and purification of a protein. The protein chosen for the experiment was an amylase, specifically beta-amylase. Amylase protein can be typically found in plants and animals. The role of this protein is to hydrolyze organic materials, such as starch and dextrin, in order to form glucose, maltose, and limit dextrin. One distinction between beta amylase and alpha amylase is that beta amylase is not found in animal. It is found primarily in plants, bacteria,…

Through both simple and fractional distillation an equimolar solution of ethyl acetate and propyl acetate was seperated. After distilling 20 mL of the mixture the samples were collected in fractions, which were then analyzed using gas chromatography. The mole ratios of the distillations were converted to a percentage to analyze percent composition. Simple distillation yielded the following results: S1- 69.88% EA/ 30.12% PA, S2- 66.73% EA/ 33.27% PA, and S3- 46.42% EA, 53.58% PA. Fractional…

A new, specific, rapid gas chromatography-mass spectrometry (GC-MS) method was described to identify and quantify pregabalin in human plasma. Silylation and acylation reaction were tried for derivatization of pregabalin before GC-MS analysis. Derivatization was achived using N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA). Identification and quantification of a new derivative were performed in selected ion monitoring mode using electron impact ionization. Pregabalin was…

1.5 REVIEW OF LITERATURE 1.5.1 Phytoconstituents of Enicostemma littorale Blume and its medicinal properties Enicostemma littorale Blume contains secondary metabolites such as alkaloids, catechins, saponins, sterols, triterpinoids, phenolic acid flavonoids and xanthones. Thse secondary metabolites exhibit various biomedical properties like anti-inflammatory [29] and anticancer [30] properties. Aqueous extract of E. littorale in alloxan induced diabetic rats was also reported to possess…

approximate 100 mL of chloroform was added to the beaker to make a pourable slurry. The slurry was then poured into the column and the solvent just about the silica was drained off into a conical flask. To minimize losses, the silica slurry beaker was rinsed with more chloroform and added to the column. Next, a thin layer of acid washed sand (3mm – 4mm) was added to the column. Next, two ‘curcumin soft gels’ was puncture using a scissor and spatula. The soft gels were squeezed and added to a 50…

Analgesic drugs contain an active pharmaceutical ingredient (API) that provides it with the pharmacological properties. Active pharmaceutical ingredients consist of acetylsalicylic acid, acetaminophen, ibuprofen, or naproxen. These ingredients are also Nonsteroidal Anti-inflammatory Drugs (NSAIDs) that are non-narcotic relievers of mild to moderate pain (Medicinenet). The purpose of this experiment was to separate an active pharmaceutical ingredient from an analgesic drug using extraction with…

Gel beads are present on a column and, as the mixture (in this case liposomes) flows through the column, aspects of the mixture can be trapped via binding to, or entering, the gel beads. This occurs on a size related basis, with molecules of specific size being entrapped by the column (Ruysschaert et al., 2005). In the case of liposomes, their large size causes them to be entrapped between the gel beads…

What is Mandrax? Mandrax is an industry name of a synthetic barbiturate type drug that is made by combining a variety of chemicals which include Methaqualone, which is its active ingredient and which has sedative hypnotic properties and Diphenhydramine which is an antihistamine and also has sedative properties. Mandrax comes is a tablet form in a variety of colors such as; white, pink, purple, brown and green and is marked with the manufacturers symbol. Methaqualone was first synthesized in…