Column chromatography

Introduction: Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. It is a method used to separate molecules under the centrifugation field based on their sedimentation rate with the help of centrifugal force. It is also used to measure the physical properties of a molecule like molecular weight, density, shape, etc. If it is used for the separation of one type of materials from others is termed as preparative centrifugation and when used for…

The invention relates to formulation of Ketorolac Tromethamine and Phenylephrine Hydrochloride immediate release tablet having less disintegration time. HPLC method was developed and validated for determination of content of Ketorolac Tromethamine and Phenylephrine Hydrochloride in immediate release tablet. UV spectrophotometric method was developed and validated to study drug release profile of Ketorolac Tromethamine and Phenylephrine Hydrochloride in immediate release tablet. Background:…

4.2.3.5. Antioxidant assays in gastric tissue homogenate 4.2.3.5.1. Catalase activity Catalase activity in the gastric tissue was measured according to the procedures of Sinha (1972). One ml of homogenate solution was taken and mixed with 5 ml of phosphate buffer of pH 7.4. To this 4 ml of 0.2 M H2O2 was added and time noted. Exactly after 180 seconds of adding H2O2, a set of 1ml of reaction mixture from the above was taken and 2 ml dichromate acetic acid was added. It was then kept in a boiling…

continetalis Kitagawa; Kaurenoic acid; Phase I; Pharmacokinetics; Ultra-performance liquid chromatography-tandem mass spectrometry Abbreviations: AIC, akaike information criteria; AUC0-∞, the area under the concentration–time curve from zero to infinity; CL/F, the total body clearance for oral administration; Cmax, the maximum plasma concentration; CV, coefficient of variation; HPLC, high-performance liquid chromatography; ka, the absorption rate constant; IS, internal standard; LLE,…

What is different is the way that the database administrator has to think about data. While in a row-oriented database he thinks in terms of individual transactions, in a column-oriented database he has to think in terms of similar items derived from sets of transactions. From the indexing point of view, he has to pay more attention to the cardinality of the data, because an index is related with a subject, such as the balance…

The variable located at the left side of the diagram, the effect of malunggay and ginger root extracts is the independent variable which in being manipulated for the dependent variable, the time spent in treating the sample infected by trichophyton rubrum. The main purpose of this study is to produce an organic antibacterial treatment for Trichophyton rubrum using Malunggay (Moringa oleifera) and Ginger (Zingiber officinale). Specifically, this study aims to answer the following: 1. In which…

experiment was to synthesize, purify, and identify p-nitroacetanilide using acetanilide as the starting material. To test for the purity and identification of our desired product, we gathered data from: Thin Layer Chromatography (TLC), Melting Point (MP) range, Infrared (IR) spectrum, Gas Chromatography Mass Spectroscopy (GC-MS), and Proton Nuclear Magnetic Resonance (1H NMR). TLC plate indicated the formation of our pure product due to the difference in Rf values between starting material and…

To identify the unknown chemical mixture (#757), a TLC analysis was conducted using three standards (aspirin, acetaminophen, and caffeine). A TLC plate was prepared by drawing an origin line and solvent front line with a pencil. Three spot were labeled A, B, and C. A sample of each standard was spotted on the analytical TLC plate approximately 1cm from the bottom of the plate. It was then enclosed in a jar filled with 2ml of 95% ethyl acetate- 5% acetic acid. (Another laboratory group conducted…

Introduction The purpose of experiment was to identify an unknown analgesic drug using thin-layer chromatography (TLC). Using the thin-layer chromatography to determine the composition of the Acetaminophen, Aspirin, Caffeine and Salicylamide, then compared with unknown analgesics. Experiment Scheme Acetaminophen Aspirin Caffeine Salicylamide C8H9NO C9H8O4 C8H10N C7H7NO Figure 1. The Chemical structure of Acetaminophen, Aspirin, Caffeine…

guidance of Dr. Raquel Ruiz and involved expression tests at different concentrations of the inducing agent IPTG, large-scale growth of BL21-DE3 E. coli cells that over-express hS100A7, utilizing fast protein liquid chromatography (FPLC) with immobilized metal ion affinity and size exclusion columns, and ultimately using the purified protein product in setting up crystallization screens. I was unable to obtain any crystals of hS100A7 due to the usage of the protease inhibitor cocktail AEBSF…