Column chromatography

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  • Tlc And Column Chromatography

    Introduction Separation, a process by which the distinguishing between substances through physical or chemical means in a mixture may be carried out. A widely employed technique for this purpose is called chromatography. 1 There are many different types of chromatography, including gas-liquid, paper, column and so on. 1 Chromatography differentiates by allowing the differences in polarity, vapor pressure, solubility and so on of different substances to be observed. 1 In our endeavor, we will be using specific types of chromatography, termed Thin-Layer Chromatography (TLC) and column chromatography. Essentially, TLC is an adsorption aspect of chromatography that uses a polar stationary phase and a non-polar mobile phase, and utilizes capillary…

    Words: 1030 - Pages: 4
  • Column Chromatography Lab

    Methods Column chromatography was set up using a 60 ml syringe clamped to a ring stand. The syringe was plugged using a small amount of cotton. An Erlenmeyer flask was placed underneath the column to collect any excess material. Eleven 20 ml vials were labeled numbers 1 through 11. Silica gel was measured to 42 ml using a graduated cylinder and then moved into a 150 ml beaker. A slurry was created by adding 50 ml of hexane to the beaker containing the silica gel. The slurry was stirred for…

    Words: 877 - Pages: 4
  • Factionation Of ABE Extract By Column Chromatography Case Study

    compounds and their formulations (Sasidharan et al., 2011). According to the World Health Organization (WHO), nearly 20,000 medicinal plants exist in 91 countries. The premier steps to exploit the biologically active compound from plant resources are extraction, pharmacological screening, isolation and characterization of bioactive compound, toxicological evaluation and clinical evaluation. The aim of the present study was to characterize the bioactive compounds from ethyl acetate fraction of…

    Words: 845 - Pages: 4
  • Lab Analysis: Reverse Phase Chromatography

    HPLC column can be categorized into two types • Reverse Phase • Normal Phase 3.4.1.1. Reverse Phase Chromatography Reverse Phase system has a non-polar stationary phase over which polar solvents are used as mobile phase; it is generally used for separation of polar species. 3.4.1.2. Normal phase chromatography Normal Phase system has a polar stationary phase over which non-polar solvents are used as mobile phase; it is generally used for separation of non-polar species.[38]. A detection…

    Words: 715 - Pages: 3
  • Transformation Of Pglo Lab Report

    supernatant was glowing rather than the bacterial pellet. After observations were made, we incubated the tube containing the lysozyme and bacteria for 15 minutes at room temperature. Again, we observed the pellet and supernatant under UV light to verify that the GFP was in the solution. Purification of GFP gene using Hydrophobic Interaction Chromatography We prepped the chromatography column by shaking the column to suspend the beads and gather them at the end of the column. We removed top and…

    Words: 785 - Pages: 4
  • Body Synthesis Protocol Analysis

    A solution of butadiene and meso-tetraphenylporphyrin (100 mg, 0.162 mmol) dissolved in dichloromethane (100 ml, 1.57 mol) in a water-jacketed flask was irradiated with compact fluorescent lamps. The solution was then concentrated in vacuo and the product subjected to column chromatography to yield the product, aldehyde, and unreacted starting butadiene which could then be recycled. 1-Phenylbutadiene…

    Words: 1392 - Pages: 6
  • Essay On Liquid Chromatography

    impact the environment, including water treatment processes. The purpose of these experiments is to identify the compounds in aquatic samples from various places throughout the world. Verifying these compounds is crucial in determining how drugs of abuse effect the environment. Ultraperformance Liquid Chromatography- Tandem Mass Spectrophotometry Analysis of Stimulatory Drugs of Abuse in Wastewater and Surface Waters1 Ultraperformance liquid chromatography is the best method for analyzing drugs…

    Words: 1473 - Pages: 6
  • Bioanalysis In Biopharmacenology

    just 10^2. LBAs can detect both, active and free forms of a large molecule drug in samples. Aside from this aspect, they take a long development time of months and have low specificity may be because of its antibody cross reactivity. With all these limitations of LBAs, they can not detect diverse proteins and peptides required for biopharmaceutical industries.2 LC-MS on the other hand provides an intact analysis, with th information of a whole peptide molecule. LC-MS Bioanalysis : This…

    Words: 1888 - Pages: 8
  • Roman Architecture Style

    Among the most distinguishable aspects of their architecture are their columns. They inherited them from the Greeks and each style is generally dictated by the capital at the top of the column. The Romans used them to commemorate people, places, or even events by carving into the columns. They would decorate the columns after being built and put them into place, often taking years to finish. They had three major orders of columns the Doric order, Ionic Order, and the Corinthian Order. The Doric…

    Words: 1307 - Pages: 6
  • Gas Chromatography Lab

    Introduction: The main purpose of this experiment was to take the Gas Chromatography/ Mass Spectrum part of the “Analysis of US bills for cocaine using gas chromatography / mass spectrometry” lab and improve upon the gas chromatographic results. This would be done by finding a way to separate the peaks of cocaine and doxepin. The lab done previously showed the use of two different methods to obtain samples of cocaine and doxepin for spiked dollar bills. The two extraction methods were…

    Words: 1038 - Pages: 5
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