Chromatography

sensitive with a binding maximum in the range of pH 3.0 to 5.0 theoretically ((#3 Ninfa, Ballou, Benore). Based on theory, pH 4 that we used in this experiment should be optimum pH for albumin and phenyl red binding. Materials: • A packed gel- chromatography column with sephadex G-25 • 0.1 M Potassium Phosphate buffer at pH 6 • 0.1 M Sodium acetate buffer at pH…

chemicals coming into contact and reacting with natural organic matter (Bougeard et al., 2010). With the amounts of hazardous chemicals being in such minute quantities, a reliable detection method is required. This can be achieved through the use of chromatography and mass spectrometry. Through these methods of detection chemical compounds can be isolated and identified while giving accurate…

Risperidone (RIS), an atypical neuroleptic drug, reported to have fewer adverse effects than traditional agents, is effective in psychoses, such as schizophrenia, and other psychiatric illnesses in adults and children, including pervasive developmental disorders (PDD), autism, and attention-deficit disorder (ADD) [1–3]. It acts primarily by selective antagonism of dopamine Type 2 (D2) and serotonin Type 2 (5HT2) receptors in the brain [4–6]. Apart from minor N-dealkylation, the major pathway of…

Rituximab GP2013 and originator Rituximab” Jan Visser et al conducted a study to demonstrate the similarity among the originator rituximab and GP2013 [9]. Methods Involved: 1) Functional Bioassay 2)Cation exchange chromatography 3)Glycan analysis Functional bioassays:- Using a cell based competitive binding assay, binding of rituximab to CD20 is determined. In this, test samples are used to displace fluorescently labelled…

Protein is the building blocks of life (nlm.nih.gov). Amino Acids are the building block of protein. Amino acids bond together to make long chains, which is protein (nlm.nih.gov). Human body uses amino acids to make proteins to help the body break down food, repair body tissue, grow, and perform many other body functions (nlm.nih.gov). Amino acids can also used as a source of energy by the body (nlm.nih.gov). According to what I learned in Bioorganic lecture, the amino acid structure contains a…

Plant proteases with excellent catalytical properties perform many functions in biological systems. Vallaris solanacea, a new plant protease has been identified and screened effectively for its caseinolytic activity. This protein activity was specifically inhibited by p-chloromercuribenzoate, which showed that it is a cysteine protease. Preliminary investigations such as effect of pH and temperature on the caseinolytic activity of crude protease were done. Stability towards temperature and pH…

migrated from the distance solvent front migrated, and lastly the pigment name. If we add the chromatography liquid to our pigments, then the extract of the pigment begin to separate onto the TLC plate. Materials: 50-mL Beakers Pencil 0.5 mL Blue-green extract 2 Pipet, graduated 4 mL Chromatography solvent 2 pipet, thin-stem 0.5 mL Spinach extract…

must isolate the enzyme to be able to conclude it can be used for antibiotic use. Using homogenate, acid precipitation, ion exchange chromatography, and 2 from of gel filtration, the purification will be tested. I hypothesize these purification steps will isolate and purify the enzyme desired. Results Figure 1 shows Sephadex carboxymethyl ion exchange chromatography of a leukocyte’s cytoplasm. The 3 things being measured in this graph are: overall protein concentration,…

Purpose The purpose of this experiment was to collect three different fractions of a mixture of hexane and toluene at differing temperatures. From there, the fractions would be measured via gas chromatography to discover the percent composition of hexane and toluene in each fraction with the ultimate goal of having one fraction with mostly hexane, one with mostly toluene, and one with an even mixture of the two. Along with this, there is also the goal of redistilling one of the fractions in…

hexane layer that is collected using the separatory funnel.6 To determine the success of the experiment, percent yield was used to determine how much product was recovered, and Thin Layer Chromatography was used throughout the experiment to monitor the reaction process.6 The…