Chromatography

Task 3 P1 When using thin layer chromatography (TLC) to test for purity of aspirin it is a quick and simple method. Firstly when using the TLC method you need to have a TLC sheet and mark on the sheet 0.5cm up on the sheet and draw a line, make sure you don’t press too hard on the paper as this will disrupt the absorbent on the paper. You need to make sure the samples have been marked on the paper so you don’t get mixed up and leave a gap between the aspirin samples so they don’t run together…

biomolecule lab, we conducted the lipid chromatography experiment to separate a mixture of biomolecules from lipids. If a positive test is conducted by the end of the experiment, there should be smudges aligned vertically on the chromatography paper, or silica gel strip, indicating the separation of different lipid molecules that were present in the substance. In contrast, if a negative test is conducted there would be no smudges aligned vertically on the chromatography paper. Substances Tested…

TLC and HPTLC Introductions Thin layer chromatography (TLC) is derived from liquid chromatography where liquid serves as mobile phase and thin layer of substance on a flat late surface serves as stationary phase. The thin layer of materials act as sorbent while the mobile phase known as solvent. Solvent will send the solutes through the sorbent depends on the strength of the mobile phase as it dissolved the solute and send them up the plate against gravity ,and resistance of sorbent…

Experiment 5 Synthesis and NMR Spectroscopy of ethyl cinnamate Introduction: Wittig reactions involve the stereoselective synthesis of olefins from phosphonium ylides and aldehydes or ketones, where the carbonyl bond is converted to an alkene double bond.1 The variety of suitable reagents and the relatively mild experimental conditions facilitate their industrial applications, such as the synthesis of epoxides, esters, carotenoids and vitamin A.2,3 Specifically, the ester ethyl cinnamate is used…

Separation of plasma proteins by molecular exclusion chromatography Sebastian Prisacariu 140013826 Introduction The components of blood is broken down into 3 parts. This breakdown is consisted of: Plasma which is a yellowish fluid that helps transport blood cells around the body and contains a small supply off proteins, platelets which are blood clotting agent, red blood cells which is a carry for oxygen and remover off carbon dioxide and white cells which is the bodies defence from infection…

for this compound in medicine if it can be separated effectively from tea granules. Caffeine acts as a stimulant, making humans more mentally alert. Two methods used to separate caffeine from tea granules are Thin Layer Chromatography and High Performance Counter Current Chromatography. In TLC, a slide of glass coated in a substance can act as the stationary phase, serving as a bed for the mobile phase to move along. Samples are applied at a certain height to be carried and separated. Using…

to 67.7°C. The boiling point range for the fourth fraction was 78.8°C to 80.1°C. A gas chromatograph is a device that measures the content and the percentage of the tested sample. For the Gas Chromatography part of the experiment I injected a 2 microliter sample of each fraction into the Gas Chromatography to find the percentage of hexane, heptane, or octane is present in each solution.…

By using the techniques of simple distillation and gas chromatography to purify and determine relative proportions of volatile compounds in their gas form. Distillation could be described as a process where a mixture was heated until it began to boil, and the condensed vapor was collected. After completing the distillation process, the gas chromatography method could be used to identify the relative components of a compound in a volatile mixture. According to the analysis between the…

meso-tetraphenylporphyrin (100 mg, 0.162 mmol) dissolved in dichloromethane (100 ml, 1.57 mol) in a water-jacketed flask was irradiated with compact fluorescent lamps. The solution was then concentrated in vacuo and the product subjected to column chromatography to yield the product, aldehyde, and unreacted starting butadiene which could then be recycled. 1-Phenylbutadiene…

Cheyenne Wilson 5.05 LAB REPORT CANDY CHROMATOGRAPHY BACKGROUND INFORMATION: Paper chromatography is a widely used method of separation. The lab will show the basic techniques of paper chromatography. In this lab, the separation of the dyes used in two different kinds of candy is performed. The candy dyes may also be compared to solutions of FD&C food dyes. Purpose: The purpose of this experiment is to…