Chloroform

Thereafter, genomic DNA was obtained by the classical “phenol-chloroform” procedure for total DNA extraction. DNA was resuspended in 200 µL of buffer TE (10 mMTris-HCl; 1 mM EDTA; pH 8.0). The purity and yield of DNA extracted were evaluated by spectrophotometry at 260 and 280 nm (Epoch, Biotek Instruments, Winooski…

There are many reasons why the cosmetic industry has become a concern for the people of the United States. Many people have had diseases and cancers that can be linked to the chemicals that are placed in their personal care products and cosmetics. Little has been done for putting regulations on these chemicals being placed in personal care products until recently with the Personal Care Products Safety Act. The chemicals that are being put into cosmetics are affecting every person who uses any…

through the peep-hole but sees no one. He then goes to turn around, but before he can move an inch, the door breaks open and hits his back, sending him crashing to the floor. Someone lands on top of him, and in the struggle, places a rag doused in chloroform over his mouth. Nova finally breaks from unconsciousness to see the masked man in front of him. “What do you want?” Nova…

A woman working at a hospital in Mumbai was brutally choked with a dog chain and raped by a co-worker. The incident left her blind, deaf, and suffering from a dreadful brain injury. This is the story of Aruna Shanbaug. The woman who is now sixty-six years of age and hasn’t responded to anyone for decades since the incident. Cases like Shanbaug and many others are presented on numerous accounts every year. To be put in such a position can be challenging on its own. To be put in the same situation…

These are the household items that anyone can purchase. Industrial chemicals such as chloroform, toluene, benzene, methanol, coolants, paints, glues, and gases. These are the most dangerous, even to a first-time user. The general method of consuming solvents is called “huffing”. Initially, blood levels peak, dizziness, disorientation, light-headedness…

The Montreal Protocol on Substances that Deplete the Ozone Layer 1. Introduction to the international agreement The Montreal Protocol came into effect as a result of the Vienna Convention for the Protection of the Ozone Layer. The Convention founded ozone depletion as highly global environmental problem that required international consensus on regulating the use of CFC’s. Two years later, on September 16th 1987, the Montreal Protocol on Substances that Deplete the Ozone Layer was signed by 24…

During World War II, big quantities of Germans that were on the concentration camps were conducted painful and then killed by the Nazis’ experiments and obviously without the opinion of them. People in this period, see the Holocaust as a lack of respect for humanity, but others see it like a progress in the modern medicine. So, in this Research project I’ll search some of those that had major impact in the modern medicine. The Egyptians put a big progress too, making the potions with plants to…

a salesperson, John’s peers and co-workers realized how good he was and was offered a higher position in less than a year. Once John has the victim in control and in his property, he takes a rag filled with chloroform stuffing it into the victim’s face. Once the victim inhales the chloroform it acts within a few seconds providing a method of sufficient concentration causing a victim to take a deeper enough breath. First effect they begin to go numb, next their vision and hearing begin to fail…

cardiac muscles (Garber, 2012, para. 2). Finally, researchers have discovered that triclosan (C12H7Cl3O2) can react with free chlorine (HOCl) within the tap water supply, a form of chloroform gas (CHCl3) can be formed (US-Five, 2016, para. 6). (See graph below). When inhaled in large enough quantities, chloroform gas causes depression, liver damage and in some cases, cancer (US-Five, 2016, para. 7). These health concerns are especially troubling because the levels of triclosan in human urine…

ionization mass spectrometry. For the DNA extraction, small amount of the fecal sample was placed in a solvent that also contained glass beads. The mixture was vortexed for a few seconds and then centrifuged. The supernatant was collected and phenol, chloroform and isoamylalcohol was added. Then quantitave PCR assay was performed. A dietary assessment was done on the children followed by biochemical analyses and statistical…