Chloroform

µl SDS and mix gently  Add 50µl of ProteinaseK  Incubate at 55 degrees for one hour  Add 200µl of 5M NaCl  Add 160µl CTAB (it is viscous at room temperature therefore already pre heated)  Incubate at 65degrees for 30 minutes  Add chilled chloroform and shake vigorously  Centrifuge @8000rpm for 15 minutes at 4 degrees  Take only the aqueous phase in a new centrifuge tube and shake…

Experiment 5 Synthesis and NMR Spectroscopy of ethyl cinnamate Introduction: Wittig reactions involve the stereoselective synthesis of olefins from phosphonium ylides and aldehydes or ketones, where the carbonyl bond is converted to an alkene double bond.1 The variety of suitable reagents and the relatively mild experimental conditions facilitate their industrial applications, such as the synthesis of epoxides, esters, carotenoids and vitamin A.2,3 Specifically, the ester ethyl cinnamate is used…

medicine did they use during the war? The civil war used different types of medicines which were : Amputation; a surgical operation used to remove a hand, arm foot or leg. Anesthesia; a drug used to make patients unconscious before surgery. Chloroform; the anesthetic most commonly used . Ligature; tying ends of blood vessels in the body to stop bleeding. Morphine; a very powerful pain killing drug used during the civil war. Scalpel; a small sharp knife used by surgeons to cut through…

prepared by treating propargyl bromide with 1H-indole-3-carbaldehyde (1) in presence of potassium carbonate in dry acetone at reflux temperature [26]. Compound 2 was further reacted by heating at reflux temperature with o-phenylenediamine (OPDA) in chloroform for 6 h afforded compound 3. Compound 3 coupled with variously substituted azides by Azide-Alkyne Cycloaddition reaction to afford title compounds 4a–j. The variously substituted azides were achieved according to the literature procedure…

potent material from cannabis plants. It can be in a wax like form, or even an oil. Hashish has been around about just as long as weed itself. It is even used in rituals in Africa and Asia. Modern methods to extract cannabinoids involve alcohol, chloroform, butane and other solvents and the outcome was traded as "honey oil", "red oil", "jelly-butane hash" or similarly named concoctions - the first dabs. As of know hshish is not known to have any dangerous side effects. Although you can still…

6. SUMMARY AND CONCLUSION 6.1 SUMMARY The current study deals mainly with phytochemical and pharmacological investigation of traditionally used medicinal plant Enicostemma littorale Blume. A perusal of the literature revealed that only fragmental information was available on this plant species regarding cytoprotective potential and immunohistochemistry. In addition the exact molecular mechanism of action of this plant species active compound swertiamarin is unknown and remote. Hence this study…

Charlotte Perkins Gilman is an American short story writer, essayist, novelist, and autobiographer. One of her most famous works his her partially autobiographical, “The Yellow Wallpaper”. It was published in 1892 in New England Magazine, and was considered a very controversial piece. The story shows the mental and emotional results of the typical rest cure prescribed during that era and the narrator’s reaction to this course of treatment. The rest cure that "The Yellow Wallpaper" describes…

measurements. If the staff forget to write down any detail at all they would be punished severely. (“Medical Experiments”..) Then after they took the twins measurements most of them got an injection to the heart. This injection was made of either chloroform or phenol which would kill them instantly. If they didn’t get this shot in their heart they were put in the cabins with the other twins and given a tattoo with a special number code. This was how they identified them. (Rosenberg) A…

John Snow is a Leader, a Physician, and the founder of Modern Epidemiology. John Snow is a 19th-century reformer who used mapping to document the relationship between health outcomes and environmental conditions. Snow is most famously known discovering Anesthesia and locating the source of Cholera. Not to mention Snow is best known for his intelligence and hard work despite his harsh circumstances. As we look into John’s life every moment is worth noting. Born in York, England on March 15, 1813…

I am a Food Science major hoping to pursue my masters degree at Washington State University. I decided to go to college because I believe that higher education is the key to success. I have made pursuing my college degree in Food Science my priority. I am highly motivated and started my college career in high school completing my Associate in Arts and Sciences. I focused my courses to directly relate to my Food Science degree. I wanted to pursue food science because of the wide career…