Centrifugation

ABSTRACT The reactivity of acid base cements forming hydroxyapatite (HA) such as, β-tricalcium phosphate (β-TCP) and dicalcium phosphate dehydrate (DCPD). Amorphous calcium phosphates, prepared by precipitation from supersaturated solutions, can also react to form apatitic cements since they are thermodynamic unstable with respect to HA and have a setting reaction more independent of particle size. Calcium phosphate cement containing an antibiotic can be used for filling bone defects and to…

The most common indications for a blood transfusion include acute blood loss, coagulopathy and other anemias.2 The veterinary professional should adhere to a strict transfusion protocol, and utilize blood typing, crossmatching procedures and careful patient monitoring; which minimizes the risk of an adverse reaction and maximizes the benefits of the transfusion.2 Ideally, potential canine blood donor candidates should range in age between 1-year-old to not more than eight years of age, and be…

The inhibitor DDX3 used in our laboratory was Human shDDX3 of lentivirus, and the control group was shLuc of lentivirus, which was transfected into the cells by infection. First, BHK-21 cell was cultured to 6 cm dish and cultured to a cell density of about 12%, then remove the medium and rinse twice with 1 ml of PBS. After removing the PBS, then add lentivirus (shDDX3 and shLuc) (MOI = 50) and 3ml 2% RPMI (containing 1/1000 polybrene), then culture for 24 hours at 37 ℃ with 5% carbon dioxide…

2.1.2. Alkaline hydrolysis Alkaline hydrolysis is a simple process in which the protein is solubilized by heating followed by the addition of calcium, sodium or potassium hydroxide as alkaline agents and maintaining the temperature at a range between 25-55 °C (Pasupuleti & Braun, 2010). During alkaline hydrolysis of proteins, some amino acids like serine and threonine are damaged during alkaline hydrolysis, however, tryptophan remains intact. Also, this process causes racemization of amino acids…

In the heterotrophic production, microalgae are grown in the dark and energized by organic carbon substrates in mixing fermenters or bioreactors. With no necessity for light energy, the system can have smaller surface-to-volume ratio of the reactor. The design can be minimal and easier to control its physical conditions due to no exposure to the outside light source. However, this system requires more total operational energy than the photoautotrophic mechanisms, since this process requires the…

When a blank containing boiled (denatured) enzyme instead of an active enzyme could be used but it would not be the better blank to use in this experiment. The better blank solution would have been the one with active enzyme because it would cause the spectrophotometer to measure the absorbance of every component in the blank except the substrate, creating a 100% transmittance. This allow the spectrophotometer to read everything that was not in the blank when the actual sample is inserted in…

and 336 h after the administration. Each PFC solution was prepared in saline or distilled water. To obtain plasma sample, all the blood samples were collected in heparinized tubes and immediately centrifuged (5,000 × g, 10 min). Following the centrifugation, plasma was stored at –80 °C until analysis. Tissue samples of the liver, kidney, heart, lung, and spleen were dissected and taken immediately after taking the blood sample at the terminal time (PFOA, 24 h; PFOS, 70 day; PFHxS, 336 h) and…

alcohol and then cool it then extract with petroleum ether mixture. This results in protein precipitation which is soluble in ammonia and fat is extracted from this method. For vitamin A Hexane is used as the solvent and after the addition of water centrifugation is done which separates out layers and upper layer contains vitamin A with hexane which is then…

Isolation and Proliferation of MSCs Bone marrow derived MSCs were isolated as described previously (Baghaban Eslaminejad et al., 2008). In brief, bone marrow from deep anesthetized wistar rats was flushed out of tibias and femurs. After 2 washings by centrifugation at 1200 rpm for 5 minutes in PBS, cells were plated in 75-cm2 tissue-culture flasks with concentrations of 0.3 to 0.4 × 106 cells/cm2 in the DEMEM medium supplemented with 15% fetal calf serum. Cells were incubated in a humidified 5%…

Nowadays, caffeine becomes more and more familiar to human. People intake different amount of caffeine from different foods. It becomes part of human’s daily routine. https://www.google.com/search?q=caffeine&espv=2&biw=706&bih=567&source=lnms&tbm=isch&sa=X&ved=0ahUKEwiVmOWn1sHMAhXGuBoKHTGYAbsQ_AUIBigB#imgrc=ya-sLhS2Ae9x2M%3A Caffeine (C8H10 N4O2) a substance that has a bitter taste and is usually found in coffee, tea, soft drinks, chocolate, kola nuts, and certain medicines. It affects body’s…