Centrifugation

The experiment calls for the extraction and purification of a protein. The protein chosen for the experiment was an amylase, specifically beta-amylase. Amylase protein can be typically found in plants and animals. The role of this protein is to hydrolyze organic materials, such as starch and dextrin, in order to form glucose, maltose, and limit dextrin. One distinction between beta amylase and alpha amylase is that beta amylase is not found in animal. It is found primarily in plants, bacteria,…

Parkinson’s Disease (PD) is a progressive neurological condition of which there is currently no cure. The main pathological feature of the disease is degeneration of the dopaminergic neurons within the brain, primarily in the Pars Compacta region of the Substantia Nigra (SN). This is thought to be a result of the formation of soluble aggregates of α-synuclein known as ‘Lewy Bodies’ in the neurones of PD patients, which can be clearly seen in various regions of the brain upon post mortem…

oxidase is used in all parts to express the concentration in the enzyme reacting into ortho-quinone (Schultz, 2006). The catechol oxidase if from the potato that was used in this experiment. The enzyme was extracted as the full strength after centrifugation and used the homogenization as the supplement for all parts of the experiment (Shultz, 2006). Concentration of the enzyme or the substrate can increase the reaction rate so the enzyme being added helps clarify the meaning of that with the…

Whatman filter paper No: 2, glycerol was added at 0.75 % (w/v), and stirred for more than 15 minutes. Two of the best concentrations, 60 and 90 mg mL−1 were prepared from freeze-dried IgY for S. putrefaciens and P. fluorescens respectively. After centrifugation (10000 ×g) for 30 min, the supernatant was collected and sterilized by filtrating, through a 0.22 µm micro-filter. Finally, IgY was added to chitosan-based coating solution. The final coating solution was homogenized under aseptic…

reducing agent [5]. This group synthesized cubic silver nanoparticles by reduction of silver nitrate (AgNO3) with ethylene glycol in the presence of Polyvinylpyrrolidone (PVP) and HCl. After synthesis the cubic silver nanoparticles were isolated by centrifugation then washed with water to remove ethylene glycol and excess PVP, and finally dispersed in deionized water for storage. The synthesis of silver nanoparticles follows…

to acquire a crude homogenate containing buffers that keep the enzymes from degrading. Next, knowing that LDH was present in the cytosol, the sample was centrifuged and cytosolic enzymes including LDH were recovered from the supernatant. After centrifugation, the sample was exposed to different concentrations of ammonium sulfate in which the least soluble enzymes would precipitate out early and most soluble enzymes would precipitate late. Then, using affinity chromatography, LDH was further…

3. Preparation of microemulsion:- Microemulsion was prepared by dispersing required quantity of Aripiprazole (15 mg/ml) in appropriate quantity of oil. The mixture was homogenized and to it, accurately weighed quantity of surfactant: cosurfactant blends was added in small portion with stirring. The blends were mixed thoroughly using magnetic stirrer and to it add dropwise double distilled water with continuous stirring around 10 min. compositions of ARP microemulsion shows in Table 1. 4.…

3.3 Results and Discussion 3.3.1 Agarose Particles By microscopy observation, both ABT and CSS were activated by the Mix&GoTM solution with significant colour change on the particle surface. Seen from Figure 3-1 and 3-2, a dark green layer was formed outside the particle after treating with Mix&GoTM. By visual observation, the agarose particles turned from colourless to green, which supported the microscopy observation. The activated particles were also sent for zeta potential measurement and…

Introduction Essential oils (EOs) are aromatic volatile liquids obtained from plant materials whether they are vascular plant or nonvascular plants including; buds, flowers, fruits, seeds, leaves, twigs, bark, wood ,roots and herbs. Citrus plants constitute one of the main essential oils sources (Burt, 2004; Hammer, 1999). They belong to Rutaceae family, which originated from tropical and subtropical south-eastern parts of the world (Sawamura, 2010). Citrus species were cultivated in China long…

different sources of drugs like amphetamine, nicotine and heroin ref., all these factors could interfere with the immunoassay. This can be avoided by evaporation of the extract to dryness followed by resuspension in 1 ml methanol and was subsequent by centrifugation for 15 min. Afterward, the supernatant was diluted…