Centrifugation

It adjusted the pressure applied on the liposomes as they were sensitive so their membranes may have been disrupted, leading to contents leakage. Shortening the centrifugation time also decreased the exposure of the liposomes to this pressure, decreasing the chance of membrane disruption. The method of centrifugation e.g. swinging head versus fixed angle, may also have affected the viability of the…

satellites,minisatellites, and microsatellites largely exist in the form of tandem arrays (these elements originally got their name as "satellites" because they separated from the bulk of nuclear DNA during centrifugation). Sequences repeated in tandem are common at the centromere (where the two halves of a replicated chromosome are held together), and at or near the telomeres (the chromosome tips). Because they are difficult to sequence, sequences repeated in…

beam is passed through the stained sperm cell. This strategy has been utilized as a part of numerous configurations for more than 30 years. These strategies deal with the reason that they isolate the X and Y-sperm by utilization of centrifugation. Amid centrifugation, controlled turning causes particles in a specimen to end up sorted into layers as indicated by the thickness of those particles. Sorting isolates the more thick X-sperm from the lighter Y-sperm. One portion of the sperm regularly…

In addition, some juice may remain in the pomace of the fruit(apple), leading to incomplete extraction process. Therefore, pectinase is needed to assist juice extraction and separation of undesirable precipitate using centrifugation or sedimentation. Research suggests that the juice yield increases with the concentration of pectin enzymes. The use of pectinase is also critical in clarification process of juices while retaining the nutrients and flavor in it. Some fruits,…

degrees centigrade and then placed in a micro-centrifuge for ten minutes. After Centrifugation, the supernatant was collected and transferred into a new tube. Then 3 microliters of silica resin was added to the supernatant to bind present nucleic acids. The tube was then incubated for 5 minutes in a heat block at 57 degrees centigrade. Then Wash Buffer was added to remove contaminants. Then the collection and centrifugation of the supernatant was repeated three more times. PCR DNA Barcoding…

tubes and stored at -80°Cuntil RNA extraction. For the separation of plasma, about 2.5 ml blood was added into potassium-ethylene-diaminetetraacetic acid (K2-EDTA)coated tubes. Plasma was separated by centrifugation at 1,000xg for 10 minutes at 4˚C to remove blood cells, followed by centrifugation of the supernatant at 14,000 x g for 10 minutes at 4˚C to remove additional cellular nuceic acids attached to cell debris. The plasma was transferred into RNase/DNase-free tubes and stored at -80 ˚C…

solutions. These solutions were centrifuged 12,000rpm for 10min to remove the by-product which are not soluble in hexane solvent. The resulting QDs solutions were purified thrice by precipitation and centrifugation with a solvent combination of hexane and anhydrous ethanol (1:4 volume ratio) by centrifugation (10,000 rpm, 10 min), Finally, the benzenethiol-capped QDs were dispersed in hexane at 10 mg/mL. Synthesis of ZnO NPs ZnO nanoparticles (NPs) were synthesized from zinc acetate dehydrate…

reduction of DCIP by the SDH-FAD2 complex. Because the enzyme SDH is only found in the mitochondria, it can be concluded that pellet three contained the most mitochondria of the cell fractions. Repeating the same experiment but varying the final centrifugation speed would be a way to maximize the amount of mitochondria in pellet 3 which could then be used for further experiments. Experiment II Our hypothesis that if the cell fraction with mitochondria is exposed to an environment either too…

Methods Each trial consisted of isolating the hearts of two mice, aged anywhere between 8-15 weeks, with the strain FVB/NJ of the Mus genus. Eight trials were conducted throughout the duration of the experiment. No female mice were used for any of the trials because because the research in the lab concentrates on males, due to known differences in protein expression among the two sexes. The effect of the VU591 drug on mitochondrial KATP function in wildtype mice was measured by calculating the…

On June 30th, 1926 Paul Berg was born and raised in the Brooklyn borough of New York City. In 1943, a year after the bombings of Pearl Harbor, he graduated from high school, enrolled to New York’s City College, and enlisted in the navy. Shortly after enlisting he would be called into active duty and spent the remainder of WWII as submarine chaser. After earning his doctorate degree he worked under Arthur Kornberg at Washington University, in St. Louis and would later move with Kornberg to…