Cell culture

migration of cells in all three cells. Cell migration is the movement of cell from one area to another, in response to a chemical signal. The chemical signal is the central to achieving functions; cell differentiation, wound repair, embryotic development and the metastasis of tumors. There is an attraction between the cell and the outer membrane in order for the movement to occur. When participating in the experiment, the cell culture medium was placed in a well the cell serum was added. The…

bacteria able to produce capsule can kill a mice, where as suspension of rough colonies of bacteria devoid of capsule caused no mortality. Slime is an extracellular polymer loosely attached to the cell wall which can be easily washed off where as capsule is a compact, thick and gelatinous structure adhered to cell which is made of glycocalyx polysaccharide, glycopeptides etc.,. Capsules are usually polysaccharide in nature, may be homo/mono polymer of (glucans, dextrans, levans) or…

After the cell reaches the confluence, the various concentrations of the samples were added and incubated for 24 hours. After incubation, the sample was removed from the well and washed with phosphate-buffered saline (pH 7.4) or MEM without serum. 100µl/well (5mg/ml)…

no unknown contaminants such as bacteria or cells can compromise the procedures of the experiment (examples of such techniques include wearing the proper personal protective equipment, cleaning important surfaces with ethanol, and pipetting liquids in…

Thanks to modern medicine and scientific research, cloning is a reality and no longer science fiction. We may great advancements to Sam cell research as well, leading to cures for diseases. All of this is very different though from what Victor Frankenstein tried when he built the monster. I will first speak on cloning and all the details having to do with it. Clones are living beings that are correct hereditary duplicates. Each and every piece of their DNA is indistinguishable. Clones can…

‘Immortal Life’ in cells happened in the mid-1900s following the treatment of an African American woman named Henrietta Lacks. The cells taken from her cervix were the first of human cells to grow rapidly and reproduce many generations of cells in a culture in a laboratory. A magnificent medical miracle however has a deeper and darker side to it as explored in The Immortal Life of Henrietta Lacks by Rebecca Skloot. It is a factual based account of the life of Henrietta Lacks and her HeLa cells.…

The world of microbiology is a vast one. In theory, microbiology is the study of single celled micro organisms and viruses, that work independently of each other. These microbial cells are the backbone and fundamental unit of life. Most of the biomass present on earth today is microbial, with almost 5.3x10^30 cells present, mostly in terrestrial and oceanic subsurfaces. Within these subsurfaces, many microbial communities exist, with especially specific types of bacteria having important niches…

There are roughly thirty-seven trillion cells present in the human body. Each of these cells have a specific function to perform like propagating one’s genes or carrying oxygen towards the different parts of the body. Some of these cells make up the skeletal system which is comprised of two hundred and seven different bones. Some of these cells make up the various organs in the body like the lungs and the heart. Some of these cells are responsible for the organs responsible for procreation, the…

This can provide information about the way proteins move through cells, interact with other proteins, and implement their effects inside living cells (Stretton et al., 1998; Scott et al., 1998). Flow cytometry, specifically FACS when dealing with GFP tagged proteins, is a way to measure and sort proteins and molecules from a mixed biological sample based on…

Engineering Genetics? Genetic engineering is when a living cell’s deoxyribonucleic acid (DNA) is altered to improve it in nearly any way one wishes. Alterations may include: how the cell would look later on in life; tricking it into producing something it normally would not; and/or increasing the amount of resources produced. Even though not everyone agrees with how the research is done on genetic engineering, it does do one thing: it betters our society. Due to research of genetic engineering,…