Catalysis

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    The synthesis of Aa-g-poly(MAA) involve the optimization of various reaction conditions like initiator concentration, monomer concentration, crosslinker concentration, solvent concentration and pH. After the optimization of various reaction parameters the replications were carried-out in triplicates for the reproducibility of the results and statistical of the results obtained. It was performed using the Statistical Package for Social Science (SPSS) version 10. Statistical results of the…

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    Abstract: Catalase is an iron porphyrin enzyme present in high concentrations in humans and animals liver tissues. It is a peroxisomal enzyme that is responsible for destruction of toxic H2O2 released during certain metabolic processes.The activity of catalase can be determined by measuring the decrease in H2O2 concentration which is the substrate. Introduction: An enzyme is a large protein that acts as a biological catalyst which changes the rate of a reaction. It provides an active site…

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    Research question Even though Amylase can get denatured at high temperatures, a slight increase in temperature, should increase the rate of amylase reaction. Research title The effect of temperature on amylase activity. Word count-1025 Background research Enzymes are catalysts which speed up reactions in living organisms. Enzymes function by decreasing the activation energy for a reaction. Amylase is an enzyme found in human saliva and catalyses the hydrolysis of starch .Amylase…

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    Effects of Different Concentrations of Caffeine on Cellular Respiration in a Saccharomyces Cerevisiae Cell Valentine Bedford Red Deer College- Red Deer Results The negative control showed no release of CO2 gas. The positive controls (0.1 and 0.5) showed positive results that the caffeine did not inhibit, but increase the CO2 amount measured in the Durham tubes. The CO2 gas released peaked at the 1.5 mL concentration of caffeine, but dropped when the caffeine concentration increased to 2.0 mL.…

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    Enzymes Lab

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    The goal of this experiment was to determine the Michaelis constant (Km) and also the maximal velocity (Vmax) and the inhibition of alkaline phosphate. In order to accomplish these goals, 5 samples were used. Each sample contained different volumes of 0.2 m MPNPP (p-nitrophenylphosphate) and 0.2 M Tris-Hcl at a pH of 8.0. To each sample 0.2 mL of the enzyme studied (Alkaline Phosphatase) were added upon insertion on the spectrophotometer apparatus. With intervals of 20 seconds their absorbance…

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    Enzyme Peroxidase Essay

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    The purpose of this lab is to find the optimal pH of the enzyme peroxidase, which is found in plant and human cells as a byproduct of photorespiration, photosynthesis or cellular respiration. This is significant because an enzyme operating in nonoptimal pH could have negative effects on the cell, and could pose a health threat to the organism. Enzymes proteins that speed up chemical reactions by lowering the activation energy. They do this by acting as a template for substrate orientation,…

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    Trend The constant, 0 v/v % hydrogen peroxide solution, did not react with the catalase, therefore it was recorded as 300+ seconds and had a reaction rate of 0 seconds-1. The 0.1 v/v % hydrogen peroxide concentration recorded a reaction rate of 0.17 seconds-1. The 0.2 v/v % hydrogen peroxide concentration, recorded a lower reaction rate than the 0.1 v/v% at 0.14 seconds-1. The 1 v/v % hydrogen peroxide concentration recorded higher at 0.49 seconds-1. The 3 v/v % hydrogen peroxide concentration…

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    Yeast Enzyme Lab Report

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    Introduction: All organisms depend on the action of enzymes to carry out the reactions of life. According to the text in Campbell Biology, enzymes are biological catalysts. These globular proteins speed the rate at which metabolic processes occur by lowering the activation energy or the energy barrier required to transform reactants to products. Each enzyme is specific, each containing active sites destined for a substrate. Substrates are the reactants or starting materials of chemical…

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    Tyrosinase Lab Report

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    A chemical reaction’s rate is described by the amount of product formed. Substances that increase that rate are called catalysts. Catalysts are often proteins called enzymes. Enzymes change the pathway of the reaction between the products and the product. However, enzymes don’t alter the starting or ending points. Enzymes are effective by reducing the activation energy. The enzyme tyrosinase is located in melanocytes. These are cells that produce the pigment melanin. Melanin gives skin, eyes,…

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    Simulating Filtration Lab

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    Abstract: Using the PhysioEx.9.1 software, I ran Activity 4: Simulating Filtration lab to learn about the filtration of solutes in a membrane system. This experiment was conducted using four solutes: NaCl, urea, glucose, and powdered charcoal. The goal was to learn if any of these solutes passed through the three different dialysis membranes at varying pressures. Does increasing the pore size increase filtration rate? What effect will pressure have on the filtration rate? These are the…

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