Catalysis

In this research, mesoporous material KIT-6 silica with high surface area (924.5 m2/g) and pore diameter (9.71 nm) was successfully synthesized using rice husk ash as a silica source. According to the SAXS analysis and TEM micrographs, the silicate structure of as-prepared KIT-was not distrupted by the functionalization and activation treatments with 3-APTES and glutaraldehyde, but showed a significant decrease in pore diameter and total pore volume as the compound was covalently bonded to the…

Use of microbial platform overexpressing such an ideal O-methyltransferase having flexibility in its catalytic activity over different classes of substrates is of great importance in producing variety of methylated natural products. With this concept, we used the aforementioned natural compounds as substrate for biotransformation reaction. Culture of recombinant E. coli strain was prepared and induced as explained in materials and method. In vivo reaction mixtures analysis of different…

Aim: This is an investigation to establish the effect of different temperatures (from 0°C -100°C) on the production of oxygen gas in a potato catalase reaction, using hydrogen peroxide as a substrate. The oxygen gas produced will be measured using a gas pressure sensor. Introduction: Enzymes are proteins, known as biological catalysts that increase the rate of reaction in a biochemical reaction without being chemically altered. The human body consists of various different types of enzymes,…

Abstract: Amylase in the body is used to break down polysaccharides into monosaccharides. The monosaccharides are simple sugars that are used to give the body energy. This effect was investigated in an experiment designed to show the breaking down of starch solution. A water bath was used to heat water up to 60oc. Pieces of dialysis tubing were wet and had starch solution put inside. Half of the measuring cylinders were filled with 60oc water, and the other half with 25oc water. Tests of the…

Recent studies have shown that direct physical contact of the human body with the surface of the earth (grounding or earthing) produces profound quantifiable physiological effects. A reasonable hypothesis to explain the benefits is that a direct connection to the earth and the human body allows the abundance of free electrons to flow from the ground to the body. It is proposed that because there is sufficient evidence to suggest that earthing has positive effects on physical health, the influx…

The objective for this lab is to construct a solubility curve for potassium nitrate in water by measuring saturation temperatures for six different solution concentrations. We form a group of four and were assigned to work on series one while the other group work on series two. Throughout the lab, we set a hot bath water temperature at 85 degree celsius which affected how potassium nitrate dissolves in water and changed the saturation temperature. In addition, our group discovered that the mass…

The rate of reaction in an enzyme catalysed reaction is also affected by the presence of inhibitors. In this experiment, the effect of temperature on reaction rate of enzyme will be studied. Peroxidase will be used as test enzyme and the variable to be studied is temperature (°C). At first, the experiment will be carried out at room temperature of 20°C. The temperature of the experimental setup will then be increased by keeping the reactants in a water bath at a temperature of 50°C. The enzyme…

An enzyme is a macromolecule that acts as a catalyst in which it speeds up reactions by lowering activation energy (Campbell 151,152). Activation energy is the energy that is required for a reaction to take place (Campbell 152). In the reactions there are substrates which are reactants that enzymes act on (Campbell 153). Enzymes bind to the substrates then creating an enzyme-substrate complex, and while the two are joined together the catalytic action of the enzyme changes the substrate to the…

DATA ANALYSIS The linear relationships between the time elapsed and the volume of gas indicates a constant enzyme reaction rate. The activity rate of catalase in figure 1 is calculated to be approximately 0.15mL/sec. The following figures show how this rate of reaction is affected by different external factors. The results in figures 2 and 5 show an increase in either enzyme concentration or substrate concentration will increase the enzyme activity. In figure 2, the 0.75% enzyme concentration…

pH and temperature amylase activity from fungal and mammal Introduction The first enzyme that was produced industrially is amylase from a fungal source in 1894, it was used to treat digestive disorder. Amylase is an enzyme that breaks down starch into sugar and starts the process of chemical digestion in human salvia. Starch breakdown of amylase has received a great deal of attention because of their technological significance and economic benefits, and is also used for the commercial…