Catalase

Unknown Bacteria 228 Adisney Pino Professor Dowding Miami Dade College Section T/R 7:05PM Introduction Enterobacter aerogenes is rod-shaped bacteria that belongs in the family of Enterobacteriaceae. This rod-shaped bacterium is gram negative which makes it a facultative anaerobe which grew perfectly in 37-degree temperature. This species was motile and therefore it can synthesize an enzyme known as ornithine decarboxylase. This bacterium is prevalent in the intestines of animals…

1) How should a urine sample be collected to ensure accurate laboratory results? As a nurse, what can you do to encourage this practice? A urine specimen can me collected in many ways: clean-catch method, by using a catheter, suprapubic, cystoscopy, and nephrostomy. Edith is a patient that could easily use the clean-catch method. This method is for patients that can understand instructions for depositing urine into a sterile cup. The female patient is to first wash her hands to avoid…

after incubation then it indicates lactose positive, but if the tube turns dark purple then its lactose negative (“Litmus Milk”, n. d). However, some more methods or test are SIM, MRVP, TSI, Nitrate Test, starch, gelatin, citrate, urea, oxidase, and catalase that all check for different thing in a bacterium. Although bacteria can have different cell structures and or ferment/metabolize different components, they also have specific shape and arrangements that can be helpful when determining a…

found together in random clusters. They are sometimes found in the same habitat as Micrococcus and can be differentiated because of the occurrence of cytochromes (5). They are typically facultative anaerobes or microaerophils and are able to produce catalase, making it possible for them to convert hydrogen peroxide to water to avoid potential damage. The major product of fermentation is lactate and they are not able to ferment citrate (4). There are several different species in the genus…

A bile esculin agar slant was inoculated with the unknown, and incubated at 37C for 24-48 hours. A 6.5% Salt Broth Test was conducted by obtaining a tube of 6.5% salt broth, inoculating the broth with the unknown, and incubating it at 37C. For a catalase test, a small amount of unknown was transferred onto a slide. A drop of H2O2 was then added to the slide, and observations were recorded. A Mannitol Fermentation test was then conducted by obtaining a Mannitol Salt Agar (MSA) plate. The unknown…

Methicillin-resistant Staphylococcus aureus, the disease also known as MRSA. This disease has been in my life since 2010. I have had an outbreak of this disease about four times since 2010. It is not a comfortable disease and I have had to live with it since then. It is very painful and a quite disgusting infection. I chose this disease because I have had plenty of personal experience from it and I know a great deal about it. I understand this disease, how it manifests and what happens when it…

ESKAPE pathogens are six organisms that bring a huge threat to human, because they are drug-resistance. Scientists have not found antibiotics that are effective since the pathogens are multi-drug resistance. Multi-drug resistance is top three on global public health that are mostly caused by nosocomial infections (hospital-acquired infection caused by bacteria, fungal and viral, parasites and other pathogens). The six pathogens are Enterococcus faecium, Staphylococcus aureus, Klebsiella…

When conducting the different tests and streaking, aseptic techniques were followed. For every T-streaked that was done, the inoculating loop was first flame for 5 seconds and was allowed to cool for 30 seconds. Afterwards the tube containing the mixed unknown bacteria was then passed over the flame. The inoculating loop was then inserted into the broth and then carefully streak on quadrant one of the plate. Afterwards, the loop was sterilized by the flame and was allow to cool for thirty…

The purpose of the unknown experiment is to understand and identify a gram positive and a gram negative. Bacteria can be very helpful in clinical uses and many pharmaceutical drugs are based on the organism. Knowing how a bacterium works and how it is structured can be crucial to determine how it affects each individual. Several biochemical tests were performing to separate and determine the identities of the microorganisms. Broth tube number 31 containing unknown microorganisms. One of the…

The most common bacterial infections that are gram positive are staphylococci which produce enzymes such as catalase which break down hydrogen peroxide bonds, bacillus cereus - food poisoning, bacillus anthracis - anthrax and clostridium tetani - tetanus. Penicillin is an antibiotic that prevents gram positive bacteria from forming peptidoglycan. Peptidoglycan plays a key role in the cell wall. If peptidoglycan was not present, then the bacterium would swell and burst due to the high internal…