Catalase

Identifying a bacteria based off of physical characteristics is almost impossible due to the commonality of shapes and physical features. Because of this, a wide range of biochemical tests were preformed in order to better identify the bacteria. Being able to identify an unknown was the most interesting part of the microbiology lab this semester, as well as, being able to preform the various tests. Proper aseptic technique was used throughout the semester and followed during all biochemical…

Energy needed for life comes from the breaking or forming of chemical bonds during chemical reactions. Energy is released in the formation of bonds and energy is also released when the bonds are broken, but in both energy is required to start the chemical reaction. This energy needed to start a chemical reaction, activation energy, comes from enzymes. Enzymes (almost always proteins) are organic catalysts that help speed up the rate of reactions and allow the reactants to get the most out of the…

We first evaluated whether Honokiol treatment was toxic to the APP-CHO cell line. Cells were treated with various concentrations of Honokiol (0.5 μM, 1 μM, 2 μM, 5 μM, 10 μM and 20 μM) for 24 h and cell survival was determined by Prestoblue® assay (Fig.1A). As shown in Fig.1A, when exposed to Honokiol concentrations of 10 μM or lower, the viability of APP-CHO cells was the same as untreated control cells. However, a significant decrease in cell viability was observed with 20 μM Honokiol…

Because bacteria are hard to grow and inoculation can take up to 14-15 hours. And incubation of up to eight weeks is needed for colonies to be observed. After incubation the following biochemical tests are performed to aid identification, it is usually catalase positive. It produces the enzyme nitrate reductase and reduces nitrate to nitrite and is therefore positive for nitrate reduction and it is niacin positive. Although the traditional culture growth and biochemical test are time consuming…

The effect of NS crude oil pretreatment on tumor volume Analysis of the data on tumor volume showed that a 4-month pretreatment with NS crude oil at the dose of 4 ml/kg/day results in significantly reduced volumes (550.46mm3±48.05; n=7) when compared to the control group (875.46.5mm3±80.17). NS1 tumor volumes (1322.83mm3±128.8; n=7) were significantly larger than the control volumes (Figure 1). There were no significant differences in mean volume of tumor tissue between NS2 and control group.…

the Voges-Proskauer test, it was interpreted that microbe B was able to produce a biochemical reaction because it turned a slight reddish-brownish copper meaning it was able to produce acetoin. In all of the bacteria, they were able to use utilize catalase. Both microbe A and microbe B had a bubbling effect while microbe C was just able to form bubbles on top of the sample. Microbe C did not produce bubbling, but was still interpreted as having a positive biochemical reaction. The oxidase test…

Introduction: Microbiology is the study of all living microorganisms like bacteria, fungi, protozoans…etc, and non-living microorganisms like viruses, viroids and prions. The significance of identifying an unknown microorganism is first to understand what type of organism it is and what structures it has to help figure out their potential benefit, danger or even neutrality to humans. Physicians benefit from this by identifying what their patients are suffering from and how to treat them. Also…

indicates the unknown gram-positive bacteria for number 18 was indeed S. aureus. This was then confirmed again by the grouping of the cell morphology (arranged in cocci cluster), the colony morphology (small pale colonies), the (+) reaction in the catalase test, as well as the (+) result for being able to ferment mannitol in the MSA plate…

The temperature at which enzyme activity is most effective is approximately at 40℃. This result was expected as this trial temperature was closest to internal body temperature, 38℃, and as catalase is present in all body organs (T. Fouad, Antioxidants). Temperature affects chemical reactions by increasing the kinetic motion of molecules and changing the rate of collisions between them. In lower temperatures, kinetic motion is decreased and there are not enough collisions between the molecules…

Streptococcus pyogenes Streptococcus pyogenes, also known as impetigo, strep throat, etc., is a flesh eating bacteria, that if left untreated, can lead to very serious symptoms. This bacterium is responsible for a wide array of infections. It can cause a sore throat, that is caused by, fever, enlarged tonsils, and sensitive cervical lymph nodes. Scarlet fever, as well as impetigo, and pneumonia are also caused by this bacterium. The less common infections consist of septicaemia, mastitis,…