Catalase

serve to counterbalance the effect of oxidants including; superoxide dismutase (SOD) catalase, Glutathione peroxidase (GSH-Px) and Glutathione GSH. (69) [201] Imbalance in the ROS production and antioxidant defense system results in interference with normal cellular processes and causing cell death. [21]. (33 rep introd) In addition, it was confirmed that ZnO NPs exhibited a dose-dependent decreasing catalase and SOD levels causing intracellular accumulation of ROS. As levels of ROS…

1. Differential diagnosis (names of possible bacteria) You should include at least 6 most relevant bacteria that can cause signs and symptoms given in the case study and rationale for including each of them (1 sentence maximum for each). An elderly man at the hospital emergency has symptoms of high temperature (at 103oF) and an above respiratory rate. Possible bacterial pathogens that could have caused his illness are: Haemophilus influenza – this bacterium, which is more susceptible to the…

Francisella tularensis is an important microbial agent which causes the severe infection of tularemia. The intracellular life cycle of this bacterium is supported by a vast range of protective guards such as enzymes. Although several enzymes are recognized in different subspecies of the bacterium, acid phosphatase and superoxide dismutase are seen in all bacterial subspecies. On the other hand, the most important enzymatic system relating to host cells is NADPH oxidase. For this reason, the…

The aim of this experiment is to determine how best to remove bacteria from an environment. Disinfectants are formulations of active chemicals that sterilize inanimate surfaces or objects, whereas antiseptics deter bacterial growth on living tissue. These agents have two modes of anti-bacterial action; growth inhibition or lethal action. Disease causing micro-organisms thrive wherever there are appropriate conditions to provide sustenance. There are four…

The experiment began with a reaction of a 1cm3 cube of lamb liver and 5mL of hydrogen peroxide solution being put in test tubes in a beaker filled with 10℃ water until the temperatures of the liver and hydrogen peroxide were 10℃ also. The liver was put into a 100mL measuring cylinder and the hydrogen peroxide was also poured in as the timer was started. After twenty seconds, several millilitres of froth had been produced from the chemical concoction. This froth was recorded into a results table,…

tests to help you determine a certain bacteria, your tests will later on lead you down a certain side to contribute in defining the genus and species. All the following procedures were carried out on the gram positive cocci unknown in this order: Catalase, Oxidase, Nitrate Reduction, Oxidation-Fermentation, Phenol Red, Bacitracin, Coagulase, and lastly, Gelatin Hydrolysis. These tests led me to believe that unknown number 93 is Kocuria rosea. Discussion Looking at the dichotomous key, the…

•Background and aim Soil salinity is one of the major environmental obstacles that limit the crop productivity. A pot experiment was conducted with an aim to explore the ameliorative effects of exogenously applied sodium nitroprusside (SNP) concentration against salinity stress in Brassica napus (L.) cv. Pactole. Methods Plants were grown under greenhouse conditions in plastic pots and were exposed to ***** mM NaCl. Further, ***** days old plants were sprayed with sodium nitroprusside (SNP,…

Aqrobacterium tumefaciens. for a long time has been doing what molecular biologists are now striving to do. It has been inserting foreign genes into plants and getting the plants to express those genes in the form of proteins. In the process the bacterium causes the plant cells to proliferate and form a gall or tumour, most commonly at or near the junction of the root and the stem. The crown gall is a neoplastic disease of plants caused by Aqrobacterium. The interest in the bacteria stems from…

containing 0.1 mM ethylenediamine tetra acetic acid and 1% (w/v) polyvinylpyrrolidone and inoculated 30 min in ice. This homogenate sample was centrifuged at 10000 rpm for 30 min at 4ºC and supernatant collected. The supernatant was assayed for Catalase (CAT), Superoxide dismutase (SOD), Malondialdehyde (MDA) and soluble protein with standardized protocol. For HM analysis, shoot and root samples were digested with 1:3 (v/v) HNO3: HCl and concentration determined by AAS. Efficiency of…

nitrate into nitrite using nitrate reductase. Thirdly, the milk agar plate T-streak remained white meaning that the unknown lacks proteases which convert casein into a transparent derivative. The next test determined that my unknown is positive for catalase. Lastly, the oxidase test resulted in a negative reaction. Meaning that my unknown is not an oxidase-producing microbe. Using Appendix E of the General Microbiology Laboratory lab handbook, I narrowed down the unknown microorganism to…