Catalase

Soil houses a vast array of life including many different types of microbes. These microbes help contribute to the ecosystems in and around the soil. For the purpose of the study of this paper the ecosystem that the sample was collected from was the forest floor. The types of microbes that grow in specific soil samples depend on many things such as pH, salinity, temperature and the variant levels of different nutrients. Moisture levels of soil and energy sources are also large factors in…

Abstract: In this lab, three variables were tested to discover their effect on the enzyme catalase, taken from a cut and blended potato. The blended potato mixture was strained and put into a centrifuge, the liquid that was in the centrifuge contained the enzyme and remained on ice. The enzyme was placed in test tubes that had varying levels of pH buffers, substrate concentrations and temperatures; the pH, substrate concentration and temperature were the three variables. The data showed that…

Abstract The aim of the present study was to investigate the effects of Cichorium intybus on lipid peroxidation activities of both enzymatic and non-enzymatic antioxidants, inflammatory mediators, myocardial enzymes and histopathology of cardiac tissues in experimental diabetic cardiomyopathy (DCM). Diabetic cardiomyopathy was induced by single intraperitoneal injection of STZ (40mg/kg) combined with high energy intake in rats. Seed extract of Cichorium intybus (CIE) (250mg/kg & 500mg/kg) was…

1991). It can successfully ferment glucose and lactose with both acid and gas. E. aerogenes is halotolerant with NaCl benefitting growth until 3% NaCl. It grows optimally at 30 degrees Celsius but will grow fully from 30-37 degrees Celsius. The catalase enzyme is present but the oxidase enzyme is absent. E. aerogenes is a facultative anaerobe. (Smalley and Kaltz,…

In order to determine the identity of two unknown bacteria, I performed a series of experiments over several days. To begin this process I created a dichotomous key that I utilized as a guide to perform a series of inoculations, while using both selective, and differential media. The series of inoculations eliminated bacterium based on test results until the correct bacteria were both positively identified. On day one of this process I received two bacteria, one labeled 7A and the other…

Enzymes and Metabolic Rates Introduction Enzymes are catalysts that speed up chemical reactions without being consumed or changed themselves. Each enzyme has a substrate that will match perfectly, almost as if it is a lock and key fit. If the substrate does not fit the enzyme only partial to no function will occur. Each enzyme is formed of polypeptide chains that wind and bend to form complex grooves and valleys. These grooves are the active sites. The active site is where the substrate will…

Desulfovibrio is gram negative bacteria. There are media that will be useful in isolating this organism. The best option for a gram negative bacteria would be using a selective media specifically MacConkey agar or EMB agar. These agars are used to isolate gram negative bacteria because the crystal violet in the MacConkey agar will not allow the growth of gram positive bacterium making it a good media to isolate Desulfovibrio. If that selective media does not work then another media is to use a…

There was a pattern revealed in the results, within the three trials of all pH levels they were all consistent numbers. Also, when the pH is acidic there will be very little oxygen produced, when the pH is neutral it will have the highest enzyme activity and when its basic it will start to produce less oxygen again. Each pH level had different concentrations, pH 4.8 which was the most acidic produced 2, 1 and 2mls of foam. pH 5.3 which is slightly acidic produced 2, 4 & 2mls of foam. pH 7.4…

Then, two more tests were conducted. The oxidase test and the catalase test. The oxidase test was done using a sterile cotton swab and an oxidase strip. First, wet a part of the strip using small amount of water and then obtain an inoculum from one of the agar slants using the wooden part of the cotton swab and apply it to the wet part of the strip. Observe for any color change within 15 seconds and interpret the results. The catalase test was performed using the slant that was the source of the…

Peggy Fender is a 59 year old woman who appears to be an alcoholic as she has had many visits to the emergency department in the past. She arrives in the afternoon appearing intoxicated and complaining of abdominal pain in the right upper quadrant of her abdominopelvic cavity. As Tim, the physician assistant proceeds with his examination he notices bruising on her arms legs and face. She is jaundiced and he notices the smell of alcohol on her breath. In addition, her liver and entire abdomen are…