Catalase

During the phase and process of the bacterial unknown project, vast amounts of test were performed in order to determine an unknown species of bacteria. Within the many procedures of identifying the unknown bacteria, I was first given a tube with the unknown bacteria, from which later on I started subcultures by streaking bacteria from the original tube onto a tryptic soy agar (TSA) slant. Throughout the process of identifying the unknown, new subcultures were produced in order to use the newest…

Introduction In this lab report I use two different techniques to identify Unknown A and Unknown B bacteria’s. These techniques are gram staining and metabolic testing. I first used Gram staining to distinguished and identify the bacteria’s. Han Christian discovered gram staining in 1882, he had biopsy a patient lung that had pneumonia. The main purpose for Gram staining is to identify if is Gram positive or Gram negative by using differential staining. While Gram staining shows the…

considered to be “facultative” based on page 85 of the lab manual appendix E (MB 352 Laboratory manual pg 37, 41, 85). Additional testing was done in lab 5, from which we utilized tests with “SIM agar deep tubes, nitrate reductase, protein hydrolysis, catalase, and cytochrome oxidase” (MB 352 Laboratory manual pg 48). The “SIM test” or “sulfide-Indole-Motility” had three parts to see “sulfide production”,…

Oxidative stress plays an important role in the development of diabetes complications. Our results demonstrate that increased production of ROS is the central and major mediator of both diabetes and diabetic gastroparesis. It is intriguing, although the production of ROS is a common mechanism, however, the activation of JNK and p38 MAPK in pylorus is unique and specific to gastroparesis. While we did not observe any ROS-mediated activation of JNK and p38 MAPK pathways in pylorus, there are…

Diclofenac sodium administration. Values are given as mean ± SD for groups of eight animals each. * Significantly different from normal group at p< 0.01 Table 2: Level of blood reduced glutathione (GSH) and activities of superoxide dismutase (SOD) and catalase (CAT) in normal and experimental groups of rats. No. Groups GSH (mg %) SOD (U/gm Hb) CAT (U/gm Hb) (I) Normal 1 % tween 80 23.64 ± 2.83 385.54 ± 18.65 95.22± 5.84 (II) Positive control Diclofenac sodium (150 mg/kg.b.w) 8.90 ± 1.77*…

Bacteria that produce catalase can be detected easily using typical store-grade hydrogen peroxide (Leboffe & Pierce, 2010). Bubbles start to form with the addition of 2 drops of hydrogen peroxide. This result concludes that unknown 16 is catalase-positive and it also produce the enzyme catalase. The unknown has the ability to live in the presence of oxygen and it was converted from hydrogen peroxide into gaseous…

Lactobacillus, Listeria, Kurthia, and Arthrobacter were eliminated because they did not match the coccoid shape of Unknown 15 (5). The catalase test was the next designated test in the identification of Unknown 15. Unknown 15 tested negative for the enzyme catalase as it did not form gas bubbles after the addition of hydrogen peroxide to the sample. Thus, all catalase…

Lowest? The highest was at pH 7, and the lowest was at pH 2. The catalase enzyme works the best at an optimal pH of 7 (neutral) and works the worst at an acidic pH of 2. How does changing the pH affect the rate of enzyme activity? Does this follow a pattern you anticipated? A change in pH not only affects the shape of…

bacteria moving towards a specific direction. The next tests utilized in determining the unknown was oxidation tests such as oxidase, catalase and nitrate reduction tests. The most pertinent of these tests in the identification process was the oxidase test which was negative, meaning the organism is a facultative anaerobic. Though both the nitrate reduction and catalase tests resulted positive, neither were a deciding factor in determining the unknown.…

Discovering Unknown 41 The overall reasoning for this experiment is to figure out what the unknown bacteria is through a process of tests and research. There are several differential media and selective & differential medias used throughout my findings, and all were done using aseptic technique. Based on the results of my test I came to a conclusion that my unknown 41 is Kocuria rosea. In order to deferiencate my unknown from the possibilities I went by the dichotomous key provided. After I…