inhibition. The size of the zones varied for each microorganism. Table 2 shows the results of the biochemical and morphological tests. The test that were performed were Sim, FTM, oxidase, catalase, MSA, blood agar, Acid fast stain and endospore stain. The sim and oxidase test were negative. The FTM, catalase, MSA and blood agar tests were positive. After the Acid Fast stain, the cells were blue and non-acid fast. After the Endospore stain, the cells were pink. This indicated that they were…
Biochemical Unknown I. Introduction Cultural Characteristics or morphology and biochemical tests can be used to identify and classify microorganism. By culturing microorganism in nutrient broth, slants, and on nutrient agar plates, the cultural characteristics or morphology can be determined. In this lab, the test tube 2 was incubated in a nutrient broth. The pigmentation of the tube was yellow. The media for test tube 2 was turbid. There were a few sediment on the bottom of the broth. In…
This is an issue because catalase will stop functioning at a smaller concentration rather than a larger one and level out at a lower rate. -By having differently soaked paper tabs, the reactions with the amounts of hydrogen peroxide will be either longer or shorter and more water…
it’s maturity level. Can grow in variety of climatic conditions and soil. Is known to be toxic to livestock. Ingestion by grazing animals causes intrahepatic cholestasis and associated liver damage. Keywords: Lantana camara, antioxidant enzymes , catalase , ascorbate peptidase , phenolics. Introduction 1. About the genera- Kingdom- Plantae…
catalyzes. However, in order for a chemical reaction to move forward, an energy barrier must be, which is precisely where enzymes are crucial. Enzymes bind to substrates, lowering the energy barrier so that the chemical reaction occurs at a faster rate. Catalase, a common enzyme that is found in almost all living organisms who are…
General Unknowns Bacterium Vitality en Vida Bacteria play a vital role in our daily lives. It is therefore our right to know the importance of being able to distinguish certain strains of bacteria. With the right knowledge we would be able to come up with a cure for a disease. An identity gives way to knowledge, to better understanding and access to information on how an organism works and how we can prevent its worsening effects or isolate its benefits. Scientists have been trying to…
Cellular Respiration takes place on the level of the cell, inside the mitochondria. This is the process of breaking down food in the presence of oxygen to form ATP. ATP stands for Adenosine Triphosphate which is an energy bearing molecule found in all living cells1. Things such as bacteria can perform this with their outer membranes. When a person runs, they use cellular respiration to create energy in the form of ATP which allows their muscles to move. Many other energy-consuming reactions of…
Enzymes are biological molecules (typically proteins) that significantly speed up the rate of virtually all of the chemical reactions that take place within cells. They are vital for life and serve a wide range of important functions in the body, such as aiding in digestion and metabolism. Some enzymes help break large molecules into smaller pieces that are more easily absorbed by the body. Other enzymes help bind two molecules together to produce a new molecule. Enzymes are highly selective…
in antioxidant capacity as a result of low urate level and decrease in rate of oxidation of more toxic arsenite (+3) to less toxic arsenate (+5) (Aposhian et al. 2003). Hydrogen peroxide is reduced to H2O by antioxidant enzymes like peroxidase and catalase [59]. In the present study, green tea has been decisively shown to restore the serum urate level with a concomitant decline in serum and tissue MDA level. The anti-ageing and life-span enhancing role of urate and SOD1 (cytosolic Cu-Zn SOD) are…
fibrin(clotting). If clotting is present, then the microbe is coagulase positive; otherwise the microbe is coagulase negative. The catalase test differentiates between microbes than can produce the enzyme catalase or not. If a microbe has the catalase enzyme, when hydrogen peroxide is added, bubbles appear making it catalase positive. If no gas or bubbles are observed, the microbe is catalase negative. A differential agar used for this…