Catalase

product of metabolism. The purpose of the catalase is to increase the speed of the decomposition of hydrogen peroxide into oxygen and water (Reviewmylife 2008). The purpose of this experiment was to determine the effect of substrate volume (H2O2), on the rate of production of oxygen. In this experiment, we use different volumes of the substrate, hydrogen peroxide, to see how it affects the rate of reaction using the potato as a source of the enzyme catalase. We can measure the rate of reaction…

indicated acid, however no bubbles had been present in the small tube. So gas is not present. Lastly I did the catalase test, this time not because of its simplicity, but because it would verify what bacteria was present. This time the catalase test was negative. It did not produce any bubbles. This confirmed that bacteria two was enterococcus faecalis, as it was the only negative catalase bacteria on our…

The purpose of performing physiological tests is to figure out certain characteristics of microorganisms or to reach a conclusion on what an unknown microorganism is. It can be also used to determine what is needed for bacteria to be cultured in or on certain media. Culture media can either be liquid or solid, and they can be used to identify certain characteristics of different bacterial species. This is indicated by the growth or lack of growth in or on certain media. Solid media can also be…

Introduction Background This experiment pertained to identifying an unknown bacterium. This bacterium was retrieved from a member of our group’s hand. Bacteria are prokaryotic, but like all other living organism there are many genus and species. Through experimentation these unknowns can be found can be brought to light. Bacteria can be classified either gram-positive or gram-negative. These groups differ by the composition of their cell walls. In particular, the difference in the composition…

it is Game positive or Gram negative, after Gram staining, there will be an ability to view the morphology or shape of this bacteria. The doctor can further test for the bacteria present, after the doctor uses gram staining, they can then use the catalase test, and polymerase chain reaction (PCR) tests to further positively identify the…

Purpose To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods 1. One test that was used to discover the identity of the given bacterial culture was the carbohydrate utilization tests. This specific set of tests involved two broths, namely phenol red lactose and phenol red sucrose, and a Durham tube placed upside down in the broth tubes. These broths can be broken down by fermentation processes,…

My journey to the microscopic world of bacteria can be likened to a child exploring the world for the first time. As the child discovers the world, she also learns several survival skills along the way. In the same manner, all the knowledge I learned from Microbiology class and laboratory sessions regarding microbial world equipped me to identify bacterial unknowns from a clinical sample. The task was to successfully separate and accurately identify two bacteria of a specimen taken from an…

then three drops of pH 4 buffer, lastly two milliliters of catalase was added to the mixture and was stirred once for two seconds, after waiting for one minute for the froth to produce, the amount of froth after one minute was seventeen millimeters. For the third part of the experiment a pH 6 buffer was used, after the measuring tube being cleaned, two milliliters of hydrogen peroxide, three drops of pH 6 buffer and two milliliters of catalase was added, the mixture was stirred once and for two…

Gram-positive cocci are an interesting group of bacteria to study. Some common species include Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus pyogenes, and Sarcina lutea. Gram-positive cocci have a gram positive cell wall, cocci morphology and many different styles of grouping. Some species have begun to exhibit a resistance to fluoroquinolone by the use of altering the drug binding site and the use of a membrane pump (Hooper 2002). Thanks to the…

ABSTRACT: The Microbiology & Immunology Laboratory Unknown Identification Project is designed to test the students’ skills and knowledge of performing biochemical tests learned during the course of the lab. Students receive a numbered tube containing a mixed culture of two unknown organisms and are required to determine the identity of each microorganism by performing a series of biochemical tests of their choice. Unknown test tube #25 was obtained and streaked onto two agar plates to obtain…