The effect that temperatures at 4°C, 23°C, 32°C, and 48°C , pH 3, 5, 7, and 9, the boiled extract, and hydroxylamine had on the peroxidase enzyme extracted from Brassica rapa. Emma O’Donoghue Fundamentals of Biology I Lab Professor William Olsen October 8, 2015 Abstract The purpose of this lab was to determine the effects that a number of factors had on enzyme activity. The enzyme used during this lab was peroxidase, which was extracted from the organism Brassica rapa. The…
Initially, sand and hydrogen peroxide were added in a test tube to create a mixture. Based on the observation conducted, no reaction was visible (i.e. no gas occurred). In result, the solution did not react because it does not contain the desired enzyme in order to break down H2O2. An enzyme is needed to decompose H2O2 into water and oxygen. With this knowledge, students were able to determine that the results for the rest of the tests did make sense. For example, the solution contained…
HALDANE RELATIONSHIP AND ONE-WAY ENZYMES INTRODUCTION: Most of the enzyme catalyzed biological reactions are reversible in nature. However, an enzyme may prefer to catalyze the reaction more efficiently in one direction over the other under given physiological concentrations of substrate or product (substrate in the reverse direction). An enzyme favouring a virtual unidirectional catalysis of an overall reversible reaction can’t escape the laws of thermodynamics. As a catalyst doesn’t change…
hydrogen peroxide into a oxygen and water (2H2O2 ----> 2H2O + O2). When this reaction occurs in the experiment, then it will be seen that oxygen gas bubbles escape and create foam like. From here, we would like to see how temperatures that occur in the environment affect a reaction of an enzyme towards a substrate. Research question How does different temperature (20°C, 30°C,40°C,50°C,60°C) affect the catalese enzyme reaction rate present in a liver, having a substrate as hydrogen…
product is known as benzoyl peroxide, which works primarily as a peeling agent, it increases the rate at which your skin sheds and also contains antibacterial properties to fight the microbes responsible for acne formation. While benzoyl peroxide does its job and does its job well in some cases, many individuals experience side-effects sometimes worse than the acne condition itself, such as itching, burning, extreme dry skin, redness and sometimes swelling. Benzoyl peroxide also promotes the…
apply it to the wet part of the strip. Observe for any color change within 15 seconds and interpret the results. The catalase test was performed using the slant that was the source of the inoculum used for the oxidase test. A drop of 3% hydrogen peroxide was added to the said slant and the tube was observed for any…
The Effects the Decomposition of Hydrogen Peroxide An investigation into how the amount of oxygen is produced by changing one variable effects the decomposition of Hydrogen Peroxide in the presence of the enzyme. This shows us that when the H2O2 has reacted with the catalyst this is what it produces. There are many ways in which the amount of oxygen produced could be affected. I believe that, surface area of the chip, temperature of liquid that is reacting, the concentration of the liquid…
In recent studies, cyanide poison is a toxic substance that appears in different variety of crops. According to Ogundele and Caxton- Martins," Cassava is the most widely consumed of these plants and it has been associated with economic condition in certain parts of the World; especially the tropics and sub tropics." These plants are called cyanophoric plants because they contain phytotoxins. Cyanide is very highly reaction to alkali earth metal. They mostly reactive to salt such as potassium,…
the chicken liver and the hydrogen peroxide was quite fast as opposed to the potato and hydrogen peroxide which was at a slow to a moderate rate. This is most likely due to the fact that cellular respiration occurs more often in animals than plants, meaning that they would have a higher ammount of catalase. 2. Test tube 5 had a greater reaction rate than test tube 4. In test tube 4, there was a higher concentration of catalase (enzymes) than hydrogen peroxide (substrates). Even though the…
According to PubChem (2015), hydrogen peroxide (34.01468 g/mol) consists of two hydrogen atoms and two oxygen atoms that can be seen in liquid and powder form. The liquid form is visible to the human eye as a colorless liquid with a clear odor that could be described as irritating or distinct…