Capillary electrophoresis

This is due to the highly polymorphic nature of mitochondrial DNA. 2.3 DNA extraction, amplification, and sequencing Each region sequenced was approximately 800 base pairs long. DNA was extracted and then amplified using PCR. Finally, capillary electrophoresis was performed to visualize the amplified PCR product. 2.4 Phylogenetic analyses There were multiple softwares used in the analysis of spider monkey phylogeny. Muscle version 3.6 was used to align sequences, while Modeltest 3.7 and…

Forensic Genetics To understand what Forensic Genetics is you first have to know what each term means. The Merriam-Webster Dictionary (2015) states that Forensics is the application of scientific knowledge and/or methods to legal problems and in solving crimes. Forensics is used in many different ways, from identify people through DNA or fingerprints to examining hair and different substances. It has been around for a long time, with some methods dating back to the 13th century (Kennedy 2013).…

Otto Schneider Grade 10 Biology Ms Ruebe D Assessment - DNA Technology DNA Profiling Forensic Identification Forensic Identification refers to the use of forensic science to identify objects from trace evidence found on them. Trace evidence is used to reconstruct crimes or accidents. DNA profiling is a method in forensic science which can identify individuals by their DNA profiles. DNA profiles are encrypted sets of letters that represent a person’s DNA makeup. These sets can be used as a…

It is mainly used for identification of organic and inorganic substances found at the scenes of crimes. Some of the sub-sections include paper chromatography, electrophoresis, and DNA analysis. Paper chromatography in forensics is used to identify different kinds of drugs, food, and other substances. This is done by putting solutes in a mobile phase through a stationary phase by way of a solvent. This separates substances…

IONIC LIQUIDS Introduction:- Ionic liquids are actually salts that are liquid in state, normally referred to compounds that have melting point below an arbitrary temperature i.e. 100° C. Unlike some other ordinary liquids like water and gasoline, that are composed of neutral species while ionic liquids are composed of ionic species and ionic intermediates commonly known as “ionic fluids” and “liquid electrolytes”. In ionic liquids there is a species that has a delocalized species and the other…

thickness. The coated plates were activated by incubating at 100ºC for 3h prior to use. The sample and positive control (Vinblastine, Sigma, 1mg/ml) were spotted on to activated TLC plate just 1cm above the subordinate edge of plate with the help of capillary tube and allowed to air dry. Simultaneously, the TLC chamber was saturated with different solvent systems (Binary and tertiary) consisting of mixture of solvents of different polarities and ratios for 20 min. The TLC plate was kept in…

physical properties such as polymerization, contrasting solubility and molecular stability. This leads to the erythrocytes becoming sickle-shaped and not able to bind oxygen efficiently. Sickled red blood cells can clog small blood vessels and capillaries, resulting in a lack of oxygen for the tissues. That can cause chronic pain, tissue infections and damage of organs. Protein…

Introduction Sickle cell disease is a genetic disorder that can be best described as irregular shaped red blood cells that block blood flow, which cause chronic periodic episodes of pain. Sickle Cells affects over 72,000 Americans and millions throughout the world, Sickle cell most commonly affect African American descent, approximately 1 in 12 African Americans carry the trait for Sickle cell and 1 of every 350 African-American infants born have the disorder and the incidence of the disorder…

The famous discussion of nature vs. nurture was somewhat interrupted by the realization that both aspects, genetics and environment, have important input in individuals. By combining the studies of genetics and psychology it is possible to correlate results from the fields and search for possible, and hopefully specific, genes involved in the expression of certain traits. For instance, Poulin et al (2011) explored the relationship between receptor genes of oxytocin and vasopressin and prediction…

treatment of hypertension. It has a more potent active metabolite (II, 2-n-butyl-4-chloro-1-[2- (1H-tetrazol-5 yl) biphenyl- 4-yl) methyl] imidazole-5- carboxyl acid)[1]. The determination of Losartan has been carried out in tablets by HPLC, capillary electrophoresis and super-critical fluid chromatography[2,3], in urine by gas chromatography- mass spectrometry[4] and, simultaneously with its active metabolite in biological fluids, by HPLC[5- 10]. Amlodipine Besylate (ADB) )[FIG.1.B],…